Circulating CD2+ monocytes are dendritic cells.
Circulating CD2+ monocytes are dendritic cells.
复制标题
DOI：
10.4049/jimmunol.163.11.5920
复制
发表时间：
1999-12
影响因子：
4.4
通讯作者：
K. Crawford;D. Gabuzda;V. Pantazopoulos;J. Xu;C. Clément;E. Reinherz;C. Alper
中科院分区：
文献类型：
--
作者：
K. Crawford;D. Gabuzda;V. Pantazopoulos;J. Xu;C. Clément;E. Reinherz;C. Alper
在单核细胞、巨噬细胞和树突状细胞的亚群上已经描述了低水平的CD 2。CD 2在约三分之一的循环单核细胞上表达，其水平比T或NK细胞上低半个对数，占PBMC的2-4%。流式细胞仪分析CD 2+和CD 2-单核细胞显示粘附分子（CD 11 a/B/c）、II类抗原（HLA-DR、-DQ、-DP）、髓样抗原（CD 13、CD 14、CD 33）或共刺激分子（CD 80、CD 86）的表达无显著差异。新鲜分离的CD 2+和CD 2-单核细胞形态相差显微镜难以区分。然而，扫描电子显微镜显示大的突出皱褶的CD 2+单核细胞上的小旋钮样的CD 2-单核细胞上的投影。培养2天后，CD 2+单核细胞大部分失去CD 14表达并形成明显的树突，而CD 2-单核细胞保留表面CD 14并保持圆形或椭圆形。新鲜分离的CD 2+单核细胞更有效的诱导剂的同种异体MLR和更有效地诱导幼稚T细胞的增殖，在HIV-1 gp 120的存在下比CD 2-单核细胞。在GM/CSF和IL-4存在下培养后，CD 2+单核细胞在诱导同种异体T细胞增殖方面比单核细胞衍生的树突状细胞或CD 2-单核细胞的效力高40倍。这些发现表明，循环中的CD 2+和CD 2-单核细胞分别是树突状细胞和巨噬细胞的前体。因此，树突状细胞在血液中比以前认为的要丰富得多，并且它们和巨噬细胞的前体作为表型、形态和功能上不同的单核细胞群体存在于循环中。
Low levels of CD2 have been described on subsets of monocytes, macrophages, and dendritic cells. CD2 is expressed on about one-third of circulating monocytes, at levels one-half log lower than on T or NK cells, representing 2-4% of PBMC. FACS analysis of CD2+ and CD2- monocytes revealed no significant difference in the expression of adhesion molecules (CD11a/b/c), class II Ags (HLA-DR, -DQ, -DP), myeloid Ags (CD13, CD14, CD33), or costimulatory molecules (CD80, CD86). Freshly isolated CD2+ and CD2- monocytes were morphologically indistinguishable by phase contrast microscopy. However, scanning electron microscopy revealed large prominent ruffles on CD2+ monocytes in contrast to small knob-like projections on CD2- monocytes. After 2 days of culture, the CD2+ monocytes largely lost CD14 expression and developed distinct dendrites, whereas the CD2- monocytes retained surface CD14 and remained round or oval. Freshly isolated CD2+ monocytes were more potent inducers of the allogeneic MLR and more efficiently induced proliferation of naive T cells in the presence of HIV-1 gp120 than did CD2- monocytes. After culture in the presence of GM/CSF and IL-4, CD2+ monocytes were up to 40-fold more potent than monocyte-derived dendritic cells or CD2- monocytes at inducing allogeneic T cell proliferation. These findings suggest that circulating CD2+ and CD2- monocytes are dendritic cells and the precursors of macrophages, respectively. Thus, dendritic cells are far more abundant in the blood than previously thought, and they and precursors of macrophages exist in the circulation as phenotypically, morphologically, and functionally distinct monocyte populations.