Systemic inflammation, adipose tissue tumor necrosis factor, and leptin expression
Systemic inflammation, adipose tissue tumor necrosis factor, and leptin expression
复制标题
DOI：
10.1038/oby.2003.74
复制
发表时间：
2003-04-01
期刊：
影响因子：
--
通讯作者：
Salas-Salvadó, J
中科院分区：
文献类型：
--
作者：
Bulló, M;García-Lorda, P;Salas-Salvadó, J
目的：探讨肿瘤坏死因子 a (TNFα) 和瘦素脂肪组织表达与低度全身炎症之间的联系，并确定炎症与肥胖程度、是否存在 2 型糖尿病以及其他心血管危险因素之间的关系。 研究方法和程序：将 91 名女性（BMI 19 至 65 kg/m(2)）分为 CRP 的三分位数。使用HOMA方法计算胰岛素抵抗。测量血清和血浆样本中的白蛋白、纤维蛋白原、C-反应蛋白 (CRP)、白介素-6、sTNFR1、sTNFR2 和瘦素水平。采用逆转录聚合酶链式反应测定腹部皮下脂肪组织样本中TNFa和瘦素的表达。结果：CRP与BMI和肥胖上部分布呈正相关。 TNFa 和瘦素脂肪组织表达在 CRP 的上三分位数中较高。此外，炎症程度最严重的患者外周可溶性 TNFR 和瘦素水平较高。糖尿病、血脂异常和高血压在 CRP 较高三分位数的患者中最为常见。即使在调整了体脂差异后，糖尿病女性的炎症标志物与非糖尿病女性也存在显着差异。 BMI、2 型糖尿病和脂肪 TNFa mRNA 水平是血清 CRP 水平的显着预测因子（r(2) = 0.28，p < 0.001）。讨论：这些结果与脂肪组织 TNFa 和瘦素的合成可以诱导白细胞介素 6、CRP 和其他急性期反应物的产生，从而有助于维持与肥胖和肥胖进展相关的慢性低度炎症状态的假设一致。其相关的合并症。
Objectives: To explore the links between tumor necrosis factor a (TNFalpha) and leptin adipose tissue expression and low-grade systemic inflammation and to determine the relationship between inflammation and the degree of adiposity, the presence of type 2 diabetes, and other cardiovascular risk factors.Research Methods and Procedures: Ninety-one women (BMI 19 to 65 kg/m(2)) were divided into tertiles of CRP. Insulin resistance was calculated using the HOMA method. Albumin, fibrinogen, C-reactive protein (CRP), interleukin-6, sTNFR1, sTNFR2, and leptin levels were measured in serum and plasma samples. TNFa and leptin expression were measured by reverse transcription-polymerase chain reaction in abdominal subcutaneous adipose tissue samples.Results: CRP was positively related to BMI and upper distribution of adiposity. TNFa and leptin adipose tissue expression were higher in the upper tertile of CRP. Also, peripheral levels of both soluble TNFRs and leptin were higher in patients with the greatest inflammation degree. Diabetes, dislipidemia, and hypertension were most prevalent in patients in the upper CRP tertile. Inflammatory markers of diabetic women were significantly different from those of nondiabetic women, even after adjusting for differences in body fat. BMI, type 2 diabetes, and adipose TNFa mRNA levels were significant predictors of serum CRP levels (r(2) = 0.28, p < 0.001).Discussion: These results are in agreement with the hypothesis that the synthesis of adipose tissue TNFa and leptin could induce the production of interleukin-6, CRP, and other acute-phase reactants, thus contributing to the maintenance of chronic low-grade inflammation state involved in the progression of obesity and its associated comorbidities.