Analysis of the carbohydrate binding specificity of the mushroom Pleurotus ostreatus lectin by surface plasmon resonance
Analysis of the carbohydrate binding specificity of the mushroom Pleurotus ostreatus lectin by surface plasmon resonance
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DOI：
10.1016/j.ab.2004.09.029
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Kawagishi, H
中科院分区：
文献类型：
--
作者：
Kobayashi, Y;Nakamura, H;Kawagishi, H
利用表面等离子体共振技术分析了平菇凝集素(POL)与糖结合的特异性。将凝集素固定在传感器芯片上，并将血凝抑制试验中最有效的抑制剂之一--亚洲牛颌下粘蛋白(asialo-BSM)与凝集素紧密结合。用共注射的方法评价不同单糖或寡糖与凝集素的结合特异性。注射一些半胱氨酸糖可促进asialo-BSM的解离。共注射实验中各糖促进解离能力的大小顺序与血凝抑制法评价的各糖的抑制能力大小顺序基本一致。总之，POL识别半乳糖残基，并且通过用岩藻糖基或乙酰氨基取代半乳糖残基的C-2位来提高特异性。用共注射的方法分析糖-凝集素结合的特异性是有效的。(C)2004 Elsevier Inc.保留所有权利。
The sugar binding specificity of the mushroom Pleurotus ostreatus lectin (POL) was analyzed by surface plasmon resonance. The lectin was immobilized to a sensor chip, and asialo-bovine submaxillary mucin (asialo-BSM), one of the most potent inhibitors in the hemagglutination inhibition assay, tightly bound to the lectin. The binding specificity of various mono- or oligosaccharides to the lectin was evaluated by the coinjection method. The dissociation of asialo-BSM was promoted by injection of some haptenic saccharides. For the most part, the order of acceleration ability of the sugars to the dissociation in the coinjection experiment agreed with that of the inhibitory potency of each sugar evaluated by the hemagglutination inhibition assay. In conclusion, POL recognized a galactosyl residue, and the specificity was increased by substitution at the C-2 position of the galactosyl residue with a fucosyl or acetylamino group. This method using the coinjection method proved useful in analysis of carbohydrate-lectin binding specificity. (C) 2004 Elsevier Inc. All rights reserved.