Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharide-challenged piglets
Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharide-challenged piglets
复制标题
日粮补充 N-乙酰半胱氨酸可减轻脂多糖挑战仔猪的肝损伤
DOI：
10.1017/s0007114513002171
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发表时间：
2014-01-14
影响因子：
3.6
通讯作者：
Wu, Guoyao
中科院分区：
文献类型：
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作者：
Yi, Dan;Hou, Yongqing;Wu, Guoyao
本研究旨在确定n -乙酰半胱氨酸（NAC）是否能调节脂多糖（LPS）应激仔猪模型的肝损伤。为此，将18头仔猪随机分为对照组、LPS组和NAC组。对照组和LPS组饲喂基础饲粮，NAC组饲喂在基础饲粮中添加500 mg/kg NAC的试验饲粮。在试验第10、13和20天，LPS处理和nac处理仔猪分别腹腔注射LPS （100 μg/kg体重），对照组仔猪腹腔注射等量生理盐水。试验第20天，LPS或生理盐水注射后3 h采血。第21天，宰杀仔猪，采集肝脏样本。饲粮中添加NAC可减弱lps诱导的肝脏组织形态学异常。与对照组相比，lps刺激仔猪血浆丙氨酸转氨酶和天冬氨酸转氨酶活性显著升高，H2O2、TNF-α、IL-6和PGE2浓度显著升高，血浆超氧化物歧化酶活性和肝脏谷胱甘肽过氧化物酶活性显著降低。LPS刺激也增加了腺苷酸的浓度和AMP:ATP的比值，但降低了腺苷酸的能量电荷以及ATP和ADP的水平。这些不利影响的LPS挑战是改善NAC补充。此外，NAC抑制lps诱导的肝热休克蛋白70和NF-κB蛋白丰度的升高。综上所述，饲粮中添加NAC可通过减少促炎细胞因子的分泌、提高抗氧化能力和改善能量代谢来减轻lps诱导的肝损伤。
The present study was carried out to determine whether N-acetylcysteine (NAC) could modulate liver injury in a lipopolysaccharide (LPS)-challenged piglet model. For this purpose, eighteen piglets were randomly assigned to the control, LPS or NAC group. Piglets in the control and LPS groups were fed a basal diet, whereas those in the NAC group were fed the basal diet supplemented with 500 mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS- and NAC-treated piglets were intraperitoneally administered LPS (100 μg/kg body weight), while the control group was administered the same volume of saline. On day 20 of the trial, blood samples were obtained 3 h after LPS or saline injection. On day 21, the piglets were killed to collect liver samples. Dietary NAC supplementation attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, in the LPS-challenged piglets, the activities of alanine aminotransferase and aspartate aminotransferase and the concentrations of H2O2, TNF-α, IL-6 and PGE2 were dramatically increased in the plasma and the activity of superoxide dismutase in the plasma and that of glutathione peroxidase in the liver were significantly decreased. The LPS challenge also increased the concentration of AMP and the ratio of AMP:ATP, but decreased adenylate energy charges and the levels of ATP and ADP. These adverse effects of the LPS challenge were ameliorated by NAC supplementation. Moreover, NAC inhibited the LPS-induced increases in the abundance of liver heat shock protein 70 and NF-κB proteins. In conclusion, these results suggest that dietary NAC supplementation alleviates LPS-induced liver injury by reducing the secretion of pro-inflammatory cytokines, increasing the antioxidative capacity and improving energy metabolism.