Pooling samples:: the key to sensitive, specific and cost-effective genetic diagnosis of Chlamydia trachomatis in low-resource countries
Pooling samples:: the key to sensitive, specific and cost-effective genetic diagnosis of Chlamydia trachomatis in low-resource countries
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DOI：
10.2340/00015555-0196
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发表时间：
2007-01-01
影响因子：
3.6
通讯作者：
Domeika, Marius
中科院分区：
文献类型：
--
作者：
Shipitsyna, Elena;Shalepo, Kira;Domeika, Marius
本研究的目的是比较用于筛查和诊断沙眼衣原体的宫颈内膜样本的性能特征和成本效益，并调查俄罗斯列宁格勒州妇女沙眼衣原体感染的患病率。总共对 1500 个宫颈内膜样本进行了单独测试，并分别以 5 个和 10 个样本为一组进行了汇总测试。使用了先前评估的内部诊断聚合酶链反应（PCR）测定法。 PCR 的敏感性和特异性均不受任一合并策略的影响。根据单独测试的样本以及 5 个和 10 个样本池，估计生殖器沙眼衣原体感染患病率分别为 6.6%、6.1% 和 6.0%。对于单个样本的诊断，合并策略可节省 53.3%（每个池 5 个样本）和 44.0%（每个池 10 个样本）的成本。在俄罗斯圣彼得堡，汇集样本进行沙眼衣原体 PCR 检测是一种准确且节省成本的诊断和大规模患病率研究方法。
The aims of this study were to compare the performance characteristics and cost-effectiveness of pooling endocervical samples for screening and diagnosis of Chlamydia trachomatis, and to investigate the prevalence of C. trachomatis infection in women in Leningrad Oblast, Russia. A total of 1500 endocervical samples were tested individually and when pooled in groups of 5 and 10 samples, respectively. A previously evaluated in-house diagnostic polymerase chain reaction (PCR) assay was utilized. The sensitivity and specificity of the PCR were not affected by either pooling strategy. The estimated prevalence of genital C. trachomatis infection was 6.6%, 6.1% and 6.0% based on individually tested samples, and pools of 5 and 10, respectively. For diagnosis of individual samples, the pooling strategies resulted in cost savings of 53.3% (5 samples per pool) and 44.0% (10 samples per pool). Pooling samples for PCR detection of C. trachomatis is an accurate and cost-saving approach for diagnosis and large-scale prevalence studies in St Petersburg, Russia.