Browning of Abdominal Aorta Perivascular Adipose Tissue Inhibits Adipose Tissue Inflammation
Browning of Abdominal Aorta Perivascular Adipose Tissue Inhibits Adipose Tissue Inflammation
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腹主动脉血管周围脂肪组织的褐变抑制脂肪组织炎症。
DOI：
10.1089/met.2017.0074
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发表时间：
2017-11-01
影响因子：
2.1
通讯作者：
Xu, Jin-Wen
中科院分区：
文献类型：
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作者：
Li, Run-Mei;Chen, Sui-Qing;Xu, Jin-Wen
背景：血管周围脂肪组织（PVAT）可通过分泌多种脂肪因子调节血管稳态。研究了PVAT布朗宁对其内分泌功能的影响。研究方法：在我们研究的第一部分，雄性Sprague-Dawley大鼠随机分为冷暴露（8 ℃）和24 ℃习服组。冷暴露7天后，收获肩胛间棕色脂肪组织（iBAT）、皮下白色脂肪组织、胸主动脉PVAT和腹主动脉PVAT（aPVAT）用于组织学和棕色标记基因表达分析。在第二部分中，雄性大鼠喂饲高脂饮食（HFD）10周。在第11周，大鼠进行冷暴露或不进行冷暴露。冷暴露14天后，收集aPVAT用于组织学、基因和蛋白表达分析。结果：冷暴露可通过增加UCP-1和PGC-1的表达水平而使aPVAT发生布朗宁。HFD饲养10周后，冷暴露14天仍能诱导aPVAT布朗宁。与热中性习服大鼠相比，HFD喂养的冷暴露大鼠aPVAT中TNF-α、IL-6和p-p65的表达水平显著降低。与此相反，p-AMPK表达水平增加aPVAT从HFD喂养大鼠冷暴露。结论：我们的研究表明，在HFD喂养的大鼠中，aPVAT的布朗宁降低了促炎脂肪因子的表达水平并激活了AMPK。
Background: Perivascular adipose tissue (PVAT) can regulate vascular homeostasis by secreting various adipokines. This study investigated the effects of PVAT browning on its endocrine function. Methods: In the first section of our study, male Sprague-Dawley rats were randomly divided into cold exposure (8 degrees C) and 24 degrees C acclimation groups. After cold exposure for 7 days, interscapular brown adipose tissue (iBAT), subcutaneous white adipose tissue, thoracic aortic PVAT, and abdominal aortic PVAT (aPVAT) were harvested for histological and brown marker gene expression analysis. In the second part, male rats were fed a high fat diet (HFD) for 10 weeks. In the 11th week, the rats were treated with or without cold exposure. After 14-day cold exposure, aPVAT was collected for histological, gene, and protein expression analysis. Results: Cold exposure had a browning effect on aPVAT by increasing UCP-1 and PGC-1 expression levels. After HFD feeding for 10 weeks, 14-day cold exposure was still able to induce aPVAT browning. Compared with thermoneutrality acclimation rats, TNF-, IL-6, and p-p65 expression levels were significantly lower in aPVAT from HFD-fed rats with cold exposure. In contrast, p-AMPK expression levels were increased in aPVAT from HFD-fed rats with cold exposure. Conclusions: Our study demonstrated that browning of aPVAT in HFD-fed rats lowered the pro-inflammatory adipokine expression levels and activated AMPK.