Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs
Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs
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DOI：
10.1111/j.1600-0765.2005.00799.x
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发表时间：
2005-06-01
影响因子：
3.5
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
--
作者：
Akizuki, T;Oda, S;Ishikawa, I
目的：牙周治疗的最终目的是使受损的牙周支持组织再生。虽然牙周膜细胞是牙周组织再生的基础，但将牙周膜细胞移植到牙周组织缺损处的研究还很少。我们开发了一种新的方法，将牙周韧带细胞作为一个薄片应用于缺损。本研究的目的是探讨牙周膜细胞片的应用后，在beagle dog.Methods的牙周愈合：自体牙周膜细胞从每只beagle dog的前磨牙提取。牙周膜韧带细胞片制造使用的温度响应细胞培养皿。在双侧下颌第一磨牙近中牙根颊侧表面手术造成裂开缺损。狗实验组（5个缺损），牙周膜细胞片与增强透明质酸载体应用于缺损。仅将透明质酸载体应用于对侧作为对照（5个缺损）。术后5周处死动物，制备脱钙标本。牙周缺损的愈合进行了评价组织学和histometrics.Results：没有临床症状的炎症或牙龈萎缩观察在实验组和对照组。实验组5个缺损中有3个缺损牙周组织愈合，骨、牙周膜和牙骨质形成。在对照组中，除了一个缺损外，没有观察到这样的牙周组织形成。组织学分析显示，实验组新牙骨质的形成明显高于对照组。结论：牙周膜细胞片具有再生牙周组织的潜力，可能成为一种新的牙周组织再生疗法。
Objective: The ultimate goal of periodontal treatment is to regenerate the damaged periodontal support. Although periodontal ligament (PDL) cells are essential for periodontal regeneration, few studies have reported the transplantation of periodontal ligament cells to periodontal defects. We developed a new method to apply periodontal lig ament cells as a sheet to the defect. The aim of this study was to investigate the periodontal healing after application of the periodontal ligament cell sheet in beagle dogs.Methods: Autologous periodontal ligament cells were obtained from extracted premolars of each beagle dog. Periodontal ligament cell sheets were fabricated using a temper ature-responsive cell culture dish. Dehiscence defects were surgically created on the buccal surface of the mesial roots of bilateral mandibular first molars of each. dog. In the experimental group (five defects), periodontal ligament cell sheet with reinforced hyaluronic acid carrier was applied to the defect. Only the hyaluronic acid carrier was applied to the contralateral side as a control (five defects). Fight weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Healing of the periodontal defects was evaluated histologically and histometrically.Results: No clinical signs of inflammation or recession of gingiva were observed in both experimental and control groups. In the experimental group, periodontal tissue healing with bone, periodontal ligament and cementum formation was observed in three out of five defects. In the control group, such periodontal tissue formation was not observed except in one defect. Histometric analysis revealed that the formation of new cementum in the experimental group was significantly higher than that in the control group.Conclusion: The periodontal ligament cell sheet has a potential to regenerate periodontal tissue and may become a novel regenerative therapy.