X-chromosome inactivation during spermatogenesis is regulated by an Xist/Tsix-independent mechanism in the mouse
X-chromosome inactivation during spermatogenesis is regulated by an Xist/Tsix-independent mechanism in the mouse
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DOI：
10.1002/gene.10163
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发表时间：
2002-12-01
期刊：
影响因子：
1.5
通讯作者：
Krawetz, SA
中科院分区：
文献类型：
--
作者：
McCarrey, JR;Watson, C;Krawetz, SA
在哺乳动物的雄性减数分裂过程中，单个 X 染色体的转录失活发生在生精细胞中，并且已被证明与小鼠精原细胞和精母细胞中 Xist 基因的表达一致。然而，携带被消除的 Xist 基因的雄性小鼠表现出正常的生育能力。在这里，我们检查了野生型小鼠精子发生过程中 Xist 基因座的表达，并检测到有义 (Kist) 转录本，但未检测到反义 (Tsix) 转录本。此外，我们检查了野生型和 Xist 小鼠的减数分裂和减数分裂后生精细胞中 X 连锁结构基因的表达和染色质构象，发现没有与功能性 Xist 基因缺失相关的差异。这些结果，以及 Xist(-) 小鼠初级精母细胞中形态正常的 XY 体的形成，表明在精子发生过程中 X 染色体失活不需要功能性 Xist 基因，因此该过程受到与调节雌性胚胎细胞中 X 染色体失活的机制不同的机制的调节。 (C) 2002 Wiley-Liss, Inc.
Transcriptional inactivation of the single X chromosome occurs in spermatogenic cells during male meiosis in mammals and has been shown to be coincident with expression of the Xist gene in spermatogonia and spermatocytes in mice. However, male mice carrying an ablated Xist gene show normal fertility. Here we examined expression from the Xist locus during spermatogenesis in wild-type mice and detected sense (Kist), but not antisense (Tsix) transcripts. In addition, we examined expression and chromatin conformation of X-linked structural genes in meiotic and postmeiotic spermatogenic cells from wildtype and Xist- mice and found no differences associated with the absence of a functional Xist gene. These results, along with the formation of a morphologically normal XY body in primary spermatocytes in Xist(-) mice, indicate that a functional Xist gene is not required for X-chromosome inactivation during spermatogenesis and that this process is therefore regulated by a different mechanism than that which regulates X-chromosome inactivation in female embryonic cells. (C) 2002 Wiley-Liss, Inc.