Three-dimensional growth and morphogenesis of mouse submandibular epithelial cells in serum-free primary culture.
Three-dimensional growth and morphogenesis of mouse submandibular epithelial cells in serum-free primary culture.
复制标题
小鼠颌下上皮细胞在无血清原代培养物中的三维生长和形态发生。
DOI：
10.1016/0014-4827(82)90057-x
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发表时间：
1982
影响因子：
3.7
通讯作者：
Nandi,S
中科院分区：
文献类型：
--
作者：
Yang,J;Larson,L;Nandi,S
小鼠下颌上皮细胞可以在原代培养中生长，使用胶原凝胶基质和由胰岛素、转铁蛋白、霍乱毒素和牛血清白蛋白（或FGF）组成的化学定义培养基。在不到2周的时间内，观察到持续的细胞生长导致细胞数量增加5 - 10倍。在这些添加剂的存在下，细胞团块通过向基质中延伸“星形”突起而增殖，从而产生三维生长。通过去除BSA并在含有胰岛素、转铁蛋白、霍乱毒素和FGF的基础培养基中添加皮质醇，可以调节这些生长产物的形态，形成“囊肿样”外观。简而言之，已经设计出一种持续生长的无血清培养基，并且简单地操纵补充剂可以调节胶原凝胶基质中的三维集落形态。最后，由此产生的外生物可以产生表皮生长因子（EGF），以响应双氢睾酮。
Mouse submandibular epithelial cells can be grown in primary culture using the collagen gel matrix and a chemically defined medium consisting of insulin, transferrin, cholera toxin, and BSA (or FGF). Sustained cell growth leading to a 5–10-fold increase in cell number was observed in less than 2 weeks. In the presence of these additives, clumps of cells proliferate by extending ‘star-like’ projections into the matrix, resulting in three-dimensional outgrowths. The morphology of these outgrowths can be modulated to form a ‘cyst-like’ appearance by deleting BSA and adding cortisol to the basal medium containing insulin, transferrin, cholera toxin and FGF. In brief, a serum-free medium for sustained growth has been devised and a simple manipulation of supplements can modulate the three-dimensional colony morphology in the collagen gel matrix. Finally, the resulting outgrowths can produce epidermal growth factor (EGF) in response to dihydrotestosterone.