Determination of matairesinol in flax seed by HPLC with coulometric electrode array detection
Determination of matairesinol in flax seed by HPLC with coulometric electrode array detection
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DOI：
10.1016/s1570-0232(01)00577-3
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发表时间：
2002-09-25
影响因子：
3
通讯作者：
Sontag, G
中科院分区：
文献类型：
--
作者：
Kraushofer, T;Sontag, G
本文描述了一种采用库仑电极阵列检测的高效液相色谱法测定亚麻籽中罗汉松脂素的方法。将脱脂样品加入双酚A（内标），在乙醇 - 重蒸水 - 12 M盐酸（2∶2∶1，体积比）的混合液中回流75分钟，以提取罗汉松脂素结合物并同时使其水解。提取物用流动相[250毫升乙腈 - 750毫升缓冲液（730毫升重蒸水，20毫升冰醋酸，用5 M氢氧化钠调节pH至3）]稀释，并注入高效液相色谱系统。罗汉松脂素在反相柱（Lichrospher 60 RP - Select B，250×4毫米，5微米）上与其他化合物分离，并在库仑电极阵列检测器中以0.8毫升/分钟的流速进行检测。八个电极相对于改性钯电极的电位分别设置为+150、+200、+250、+300、+350、+400、+550和+600 mV。七个样品中测定的罗汉松脂素含量在7 - 28.5微克/克之间。定量限为5微克/克。（C）2002爱思唯尔科学出版社。版权所有。
A HPLC method coupled with coulometric electrode array detection for the determination of matairesinol in flax seed is described. The defatted sample was spiked with bisphenol A (internal standard), refluxed for 75 min in a mixture of ethanol-bidistilled water-12 M hydrochloric acid (2:2:1, v/v/v) to extract matairesinol conjugates and to hydrolyze them simultaneously. The extract was diluted with mobile phase [250 ml acetonitrile-750 ml buffer (730 ml bidistilled water, 20 ml glacial acetic acid adjusted to pH 3 with 5 M sodium hydroxide)] and injected into the HPLC system. Matairesinol was separated from other compounds on a reversed-phase column (Lichrospher 60 RP-Select B, 250 x 4 mm, 5 mum) and detected in a coulometric electrode array detector using a flow-rate of 0.8 ml/min. The potentials of the eight electrodes were set on +150, +200, +250, +300, +350, +400, +550 and +600 mV against modified palladium electrodes. The content of matairesinol determined in seven samples varies between 7 and 28.5 mug/g. The limit of quantitation is 5 mug/g. (C) 2002 Elsevier Science B.V. All rights reserved.