Thrombopoietin alone or in the presence of stem cell factor supports the growth of KIT(CD117)low/MPL(CD110)+ human mast cells from hematopoietic progenitor cells
Thrombopoietin alone or in the presence of stem cell factor supports the growth of KIT(CD117)low/MPL(CD110)+ human mast cells from hematopoietic progenitor cells
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DOI：
10.1016/j.exphem.2004.12.005
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发表时间：
2005-04-01
影响因子：
2.6
通讯作者：
Metcalfe, DD
中科院分区：
文献类型：
--
作者：
Kirshenbaum, AS;Akin, C;Metcalfe, DD
目标。已知促血小板生成素(TPO)可促进血小板数量，对人巨核细胞(HuMK)具有生长促进作用，并在附加生长因子存在的情况下增加红细胞、单核细胞、肥大细胞和粒细胞的数量。我们研究了TPO单独或在干细胞因子(SCF)存在下对人肥大细胞(HuMCs)的支持能力。将CD34(+)多能细胞和CD34(+)/CD117(+)/CD13(+)的HUMC祖细胞在rhTPO中培养，并进行HuMC检测。同样，我们在干细胞因子(SCF)培养的CD34(+)细胞中加入了rhTPO，促进了HUMC的生长。当CD34(+)细胞在10 ng/mLrhTPO和10 ng/mLrhSCF中培养时，与单独使用rhSCF相比，TPO能增加HUMC的数量。高浓度的重组人TPO(50 ng/mL)与100 ng/mLrhSCF共同作用时，可抑制CD117(高)HuMCs依赖于rhSCF的亚群，而促进CD117(低)HuMCs。人CD34(+)/CD117(+)/CD13(+)细胞单独培养1~2周，分化为CD41(+)/CD110(+)HuMKs(85%~90%)和Fc epsilon RI+/CD117(Low)/CD13(+)HuMCs(5%~10%)。RhTPO诱导的HuMCs表达TPO(CD110)受体、类胰蛋白酶和糜酶，在rhSCF中再培养后存活。在重组人干细胞因子存在下，TPO对人脐静脉系膜细胞的作用因每种生长因子的相对浓度不同而不同，而TPO单独或与重组人干细胞因子联合支持CD117(低)/CD110(+)人脐静脉系膜细胞。(C)2005年国际实验血液学学会。由爱思唯尔公司出版。
Objectives. Thrombopoietin (TPO) is known to promote platelet number, have growth-promoting potential for human megakaryocytes (HuMKs), and increase erythrocyte, monocyte, mast cell, and granulocyte numbers in the presence of additional growth factors. We explored the ability of TPO alone or in the presence of stem cell factor (SCF) to support human mast cells (HuMCs).Methods. CD34(+) pluripotent and CD34(+)/CD117(+)/CD13(+) HuMC progenitor cells were cultured in rhTPO and examined for HuMCs. Similarly, we added rhTPO to CD34(+) cells cultured in stem cell factor (SCF), which promotes HuMC development.Results. When CD34(+) cells were cultured in 10 ng/mL rhTPO and 10 ng/mL rhSCF, TPO enhanced HuMC numbers compared to rhSCF alone. Higher concentrations of rhTPO (50 ng/mL) in the presence of 100 ng/mL rhSCF inhibited the rhSCF-dependent subpopulation of CD117(high) HuMCs, while promoting CD117(low) HuMCs. Human CD34(+)/CD117(+)/CD13(+) cells cultured in rhTPO alone for 1 to 2 weeks differentiated into CD41(+)/CD110(+) HuMKs (85-90%) and Fc epsilon RI+/CD117(low)/CD13(+) HuMCs (5-10%). RhTPO-induced HuMCs expressed the TPO (CD110) receptor, tryptase, and chymase and survived when recultured in rhSCF.Conclusion. The effect of TPO on HuMCs in the presence of rhSCF varies, depending on the relative concentration of each growth factor, while TPO alone or in combination with rhSCF supports a unique population of CD117(low)/CD110(+) HuMCs. (c) 2005 International Society for Experimental Hematology. Published by Elsevier Inc.