Possible approach for serodiagnosis of ascariasis by evaluation of immunoglobulin G4 response using Ascaris lumbricoides somatic antigen
Possible approach for serodiagnosis of ascariasis by evaluation of immunoglobulin G4 response using Ascaris lumbricoides somatic antigen
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DOI：
10.1128/jcm.39.8.2991-2994.2001
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发表时间：
2001-08-01
影响因子：
9.4
通讯作者：
Chatterjee, BP
中科院分区：
文献类型：
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作者：
Bhattacharyya, T;Santra, A;Chatterjee, BP
采用硫酸铵分离、凝胶渗透、离子交换、高效凝胶渗透液相色谱等层层析方法，将蛔虫体抗原纯化至均匀（分子量34 kDa）。该抗原与蛔虫感染患者的免疫球蛋白G （IgG）结合较强；在感染其他线虫的患者中与IgE和IgG有交叉反应。在63例蛔虫感染患者中，它与IgG4特异性反应（P < 0.001），占总IgG应答的65%，尽管观察到与IgG1， IgG2和IgG3亚类的交叉反应，表明该检测系统的独特特异性及其在蛔虫病血清诊断中的潜在用途。
Somatic antigen of Ascaris lumbricoides was purified to homogeneity (molecular mass, 34 kDa) by ammonium sulfate fractionation and successive chromatographic procedures, namely, gel permeation, ion exchange, and high-performance gel permeation liquid chromatographies. The antigen showed strong binding with immunoglobulin G (IgG) in Ascaris-infested patients;and was cross-reactive with IgE and IgG in patients infested with other nematodes. It reacted specifically with IgG4 (P < 0.001) in 63 Ascaris-infested patients, which represented 65% of the total IgG response, though cross-reactivity with IgG1, IgG2, and IgG3 subclasses was observed, indicating the unique specificity of this test system and its potential utility in the serodiagnosis of ascariasis.