Behavioral insensitivity to DEET in Aedes aegypti is a genetically determined trait residing in changes in sensillum function
Behavioral insensitivity to DEET in Aedes aegypti is a genetically determined trait residing in changes in sensillum function
复制标题
DOI：
10.1073/pnas.1001313107
复制
发表时间：
2010-05-11
影响因子：
11.1
通讯作者：
Field, Linda M.
中科院分区：
文献类型：
--
作者：
Stanczyk, Nina M.;Brookfield, John F. Y.;Field, Linda M.
N，N-二乙基-m-甲苯酰胺（DEET）是最有效和最常用的驱蚊剂之一。然而，在实验室试验期间，尽管存在避蚊胺，仍有一小部分蚊子被人类的气味所吸引。在本研究中，行为分析鉴定了对避蚊胺不敏感的埃及伊蚊雌性，并且在几代中选择敏感或不敏感的雌性与敏感性未知的雄性，导致两个种群中不敏感的雌性比例不同。杂交实验表明，“不敏感”性状占主导地位。触角电图显示，与选择的敏感线相比，选择的不敏感线对避蚊胺的反应降低，单感觉器记录确定了不敏感线中对避蚊胺敏感的感觉器无反应。本研究表明，埃及伊蚊对避蚊胺的行为不敏感是一种遗传决定的显性性状，存在于感觉功能的改变中。
N,N-Diethyl-m-toluamide (DEET) is one of the most effective and commonly used mosquito repellents. However, during laboratory trials a small proportion of mosquitoes are still attracted by human odors despite the presence of DEET. In this study behavioral assays identified Aedes aegypti females that were insensitive to DEET, and the selection of either sensitive or insensitive groups of females with males of unknown sensitivity over several generations resulted in two populations with different proportions of insensitive females. Crossing experiments showed the "insensitivity" trait to be dominant. Electroantennography showed a reduced response to DEET in the selected insensitive line compared with the selected sensitive line, and single sensillum recordings identified DEET-sensitive sensilla that were nonresponders in the insensitive line. This study suggests that behavioral insensitivity to DEET in A. aegypti is a genetically determined dominant trait and resides in changes in sensillum function.