Panax notoginseng Saponins Improve Erectile Function through Attenuation of Oxidative Stress, Restoration of Akt Activity and Protection of Endothelial and Smooth Muscle Cells in Diabetic Rats with Erectile Dysfunction
Panax notoginseng Saponins Improve Erectile Function through Attenuation of Oxidative Stress, Restoration of Akt Activity and Protection of Endothelial and Smooth Muscle Cells in Diabetic Rats with Erectile Dysfunction
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DOI：
10.1159/000354878
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发表时间：
2014-01-01
影响因子：
1.6
通讯作者：
Gou, Xin
中科院分区：
文献类型：
--
作者：
Li, Huan;He, Wei-Yang;Gou, Xin
三七总皂苷（Panax notoginseng saponins，PNS）是一种应用广泛的中药，具有抗氧化作用.本研究通过建立糖尿病性勃起功能障碍大鼠模型，探讨三七总皂苷对糖尿病性勃起功能障碍大鼠勃起功能的改善作用及其机制。将大鼠随机分为4组：3个PNS处理组（50、100和150 mg/kg）和1个生理盐水处理对照组。治疗4周后，对海绵体神经进行电刺激，并测量海绵体内压力以评估勃起功能。检测各组大鼠阴茎组织中丙二醛、超氧化物歧化酶和谷胱甘肽的含量。用电子显微镜检查阴茎的超微结构变化。免疫组化法检测Akt的表达。结果表明，与对照组相比，PNS处理组（100和150 mg/kg）的海绵体内压力增加。PNS组大鼠血清超氧化物歧化酶、谷胱甘肽和Akt水平升高（P < 0.05），丙二醛水平降低（P < 0.05）。电镜观察到对照组阴茎内皮细胞破裂、平滑肌细胞受损和血栓形成，但PNS组（10和150 mg/kg）未观察到。结果提示三七总皂苷能改善糖尿病大鼠的勃起功能。这种改善与Akt表达增加，抑制氧化应激以及阴茎内皮细胞和平滑肌细胞功能恢复有关。(C)2013 S. Karger AG，巴塞尔
Panax notoginseng saponins (PNS), which have an antioxidant property, are a widely used traditional Chinese medicine. In this study we investigated whether PNS can improve erectile function in rats with erectile dysfunction and the underlying mechanism by using a rat diabetic erectile dysfunction model. The rats were randomly divided into four groups: three PNS-treated groups (50, 100 and 150 mg/kg) and one saline-treated control group. Four weeks post treatment, electrostimulation was applied to the cavernous nerve and intracavernous pressure was measured to assess erectile function. Malondialdehyde, superoxide dismutase and glutathione were detected in the penises of all rats. Ultrastructural changes in the penises were examined by electron microscopy. Expression of Akt was detected by immunohistochemistry. The results showed that intracavernous pressure was increased in PNS-treated groups (100 and 150 mg/kg) compared to the control group. The levels of superoxide dismutase, glutathione and Akt were increased (p < 0.05) while that of malondialdehyde was decreased in the PNS groups. Ruptured endothelium, impaired smooth muscle cells and thrombus in the penises were detected by electron microscopy in the control group, but not in the PNS groups (10 and 150 mg/kg). The results suggest that PNS improves erectile function in diabetic rats. This improvement was associated with increased Akt expression, suppressed oxidative stress and restored functions of endothelial cells and smooth muscle cells in the penis. (C) 2013 S. Karger AG, Basel