The ginsenoside protopanaxatriol protects endothelial cells from hydrogen peroxide-induced cell injury and cell death by modulating intracellular redox status
The ginsenoside protopanaxatriol protects endothelial cells from hydrogen peroxide-induced cell injury and cell death by modulating intracellular redox status
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DOI：
10.1016/j.freeradbiomed.2009.11.013
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发表时间：
2010-02-01
影响因子：
7.4
通讯作者：
Yue, Patrick Ying Kit
中科院分区：
文献类型：
--
作者：
Kwok, Hoi Hin;Ng, Wai Yee;Yue, Patrick Ying Kit
人参皂苷是人参的有效成分，具有心血管保护作用。黄芪皂苷的作用机制被认为与其防止细胞氧化应激的能力有关。本研究旨在探讨人参原人参三醇（PPT）对过氧化氢（H2 O2）诱导的内皮细胞损伤和细胞死亡的保护作用。PPT预处理人脐静脉内皮细胞（HUVECs）24 h，能保护HUVECs免受H2 O2损伤。除细胞死亡外，PPT预处理还可减少H2 O2诱导的DNA损伤、DNA修复酶PARP-1的过度活化以及伴随的细胞内底物NAD（+）的耗竭。PPT可逆转H_2O_2引起的ATP/ADP比值下降。谷胱甘肽的代谢也发生了变化。H_2O_2可引起GSH水平显著下降，GSH/GSSG比值下降。这可以通过用PPT预处理来防止。该作用与谷胱甘肽代谢酶谷胱甘肽还原酶和谷胱甘肽过氧化物酶的活性增加有关。这些结果表明，人参PPT可以保护HUVECs免受H2 O2诱导的细胞死亡，通过其抗氧化应激的作用，这可能是人参的心血管保护作用的原因。(C)2009 Elsevier Inc. All rights reserved.
Ginsenosides, the active components of the famous Chinese herb ginseng, have been suggested to possess cardiovascular-protective effects. The mechanism of ginsenosides is believed to be associated with their ability to prevent cellular oxidative stress. The purpose of this study was to explore the cytoprotective effects of the ginsenoside protopanaxatriol (PPT) on hydrogen peroxide (H2O2)-induced endothelial cell injury and cell death. Pretreatment of human umbilical vein endothelial cells (HUVECs) with PPT for 24 h was able to protect the cells against H2O2-induced injury. In addition to cell death, pretreatment with PPT could also reduce H2O2-induced DNA damage, overactivation of the DNA repair enzyme PARP-1, and concomitant depletion of the intracellular substrate NAD(+). Furthermore, PPT could reverse the decrease in ATP/ADP ratio caused by H2O2. The metabolism of glutathione was also changed. H2O2 could induce a significant decrease in GSH level resulting in a decrease in the GSH/GSSG ratio. This could be prevented by pretreatment with PPT. The action was associated with increasing activities of the GSH-metabolizing enzymes glutathione reductase and glutathione peroxidase. These findings suggest that the ginsenoside PPT Could protect HUVECs against H2O2-induced cell death via its action against oxidative stress, which may be responsible for the cardiovascular-protective action of ginseng. (C) 2009 Elsevier Inc. All rights reserved.