Conserved regulatory state expression controlled by divergent developmental gene regulatory networks in echinoids
Conserved regulatory state expression controlled by divergent developmental gene regulatory networks in echinoids
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DOI：
10.1242/dev.167288
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发表时间：
2018-12-15
期刊：
影响因子：
4.6
通讯作者：
Peter, Isabelle S.
中科院分区：
文献类型：
--
作者：
Erkenbrack, Eric M.;Davidson, Eric H.;Peter, Isabelle S.
动物身体计划的进化是由发育基因调控网络（GRNs）的变化驱动的，但在大多数情况下，网络如何改变以控制新的发育表型仍然没有解决。在这里，我们解决GRN的进化，通过比较两个海胆，Strongylocentrotus purpuratus和Eucidaris tribuloides，至少2.68亿年的独立进化的内中胚层GRN。我们首先分析了S. purpuratus GRN在E.蒺藜胚胎，表明orthostransferase调控基因表达在相应的endomesodermal细胞的命运在这两个物种。然而，调控基因的扰动揭示了S。purpuratus GRN与E.蒺藜状。例如，中胚层Delta/Notch信号传导控制E.但控制中胚层的诱导和激活的正反馈电路在S。紫色的这些结果表明，海胆内中胚层GRN的架构演变的广泛的增益和损失的监管之间的相互作用的一组保守的调控因子，控制内中胚层细胞的命运规范。
Evolution of the animal body plan is driven by changes in developmental gene regulatory networks (GRNs), but how networks change to control novel developmental phenotypes remains, in most cases, unresolved. Here, we address GRN evolution by comparing the endomesoderm GRN in two echinoid sea urchins, Strongylocentrotus purpuratus and Eucidaris tribuloides, with at least 268 million years of independent evolution. We first analyzed the expression of twelve transcription factors and signaling molecules of the S. purpuratus GRN in E. tribuloides embryos, showing that orthologous regulatory genes are expressed in corresponding endomesodermal cell fates in the two species. However, perturbation of regulatory genes revealed that important regulatory circuits of the S. purpuratus GRN are significantly different in E. tribuloides. For example, mesodermal Delta/Notch signaling controls exclusion of alternative cell fates in E. tribuloides but controls mesoderm induction and activation of a positive feedback circuit in S. purpuratus. These results indicate that the architecture of the sea urchin endomesoderm GRN evolved by extensive gain and loss of regulatory interactions between a conserved set of regulatory factors that control endomesodermal cell fate specification.