Effects of bioirrigation of non-biting midges (Diptera: Chironomidae) on lake sediment respiration.
Effects of bioirrigation of non-biting midges (Diptera: Chironomidae) on lake sediment respiration.
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DOI：
10.1038/srep27329
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发表时间：
2016-06-03
影响因子：
4.6
通讯作者：
Krause S
中科院分区：
文献类型：
--
作者：
Baranov V;Lewandowski J;Romeijn P;Singer G;Krause S
生物灌溉或由于红虫（双翅目、摇蚊科的幼虫）等生物的活动而将液体输送到沉积物基质中，对湖泊沉积物呼吸产生重大影响。然而，以前的生物灌溉影响的摇蚊的量化一直受到技术挑战，如难以分开动物和细菌呼吸的限制。本文介绍了一种基于生物活性示踪剂刃天青的新方法，用于在恒定供氧的非密封系统中原位测量呼吸。应用这种新方法在微观实验中发现，生物灌溉提高沉积物呼吸高达2.5倍。这种新方法产生的耗氧量比以前报道的要低，因为它只对好氧异养呼吸敏感，而对其他导致氧气减少的过程不敏感。从而为定量研究沉积物中动物呼吸作用和微生物呼吸作用的无机耗氧量提供了依据。
Bioirrigation or the transport of fluids into the sediment matrix due to the activities of organisms such as bloodworms (larvae of Diptera, Chironomidae), has substantial impacts on sediment respiration in lakes. However, previous quantifications of bioirrigation impacts of Chironomidae have been limited by technical challenges such as the difficulty to separate faunal and bacterial respiration. This paper describes a novel method based on the bioreactive tracer resazurin for measuring respiration in-situ in non-sealed systems with constant oxygen supply. Applying this new method in microcosm experiments revealed that bioirrigation enhanced sediment respiration by up to 2.5 times. The new method is yielding lower oxygen consumption than previously reported, as it is only sensitive to aerobic heterotrophous respiration and not to other processes causing oxygen decrease. Hence it decouples the quantification of respiration of animals and inorganic oxygen consumption from microbe respiration in sediment.