Molecular reconstruction of Sleeping beauty, a Tc1-like transposon from fish, and its transposition in human cells
Molecular reconstruction of Sleeping beauty, a Tc1-like transposon from fish, and its transposition in human cells
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DOI：
10.1016/s0092-8674(00)80436-5
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发表时间：
1997-11-14
期刊：
影响因子：
64.5
通讯作者：
Izsvak, Z
中科院分区：
文献类型：
--
作者：
Ivics, Z;Hackett, PB;Izsvak, Z
从鱼类分离的Tc1/Mariner转座子超家族成员似乎由于突变的积累而转座不活跃。分子系统发育数据被用来构建合成转座子--睡美人，它可能与一种古老的元素相同或等效，这种元素部分通过物种间的水平传播分散在鱼类基因组中。通过消除失活突变，设计了鲑鱼亚家族转座酶基因的共同序列。睡美人转座酶以底物特异性的方式与鲑鱼转座子的反向重复序列结合，它在鱼类以及小鼠和人类细胞中介导精确的剪切和粘贴转座。睡美人是一种来自脊椎动物的活跃的DNA转座子系统，用于遗传转化和插入突变。
Members of the Tc1/mariner superfamily of transposons isolated from fish appear to be transpositionally inactive due to the accumulation of mutations. Molecular phylogenetic data were used to construct a synthetic transposon, Sleeping Beauty, which could be identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase binds to the inverted repeats of salmonid transposons in a substrate-specific manner, and it mediates precise cut-and-paste transposition in fish as well as in mouse and human cells. Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis.