Quantification of ethanol concentrations in the extracellular fluid of the rat brain: In vivo calibration of microdialysis probes
Quantification of ethanol concentrations in the extracellular fluid of the rat brain: In vivo calibration of microdialysis probes
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DOI：
10.1046/j.1471-4159.2000.0751685.x
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发表时间：
2000-10-01
影响因子：
4.7
通讯作者：
Gonzales, RA
中科院分区：
文献类型：
--
作者：
Robinson, DL;Lara, JA;Gonzales, RA
传统的微透析技术提供定性数据，尽管药效学分析通常需要定量数据。本研究评估了一种潜在的有用的体内传递技术，以校准乙醇的微透析探针。我们在受试者体内测量了2天内的体内递送浸提分数，未发现随时间的变化。我们测试了扩散方向对浸提分数的影响，发现乙醇扩散出探针的影响高于扩散进入探针的影响，无论是在体外还是在体内。体内浸提分数的扩散与扩散之比（OUT）为0.65 +/- 0.03。最后，我们预测细胞外脑乙醇浓度后，1克/公斤乙醇管理使用体内交付，“无净流量”透析，或在体内交付校正扩散方向与体内提取分数比。体内递送和“无净流量”透析预测的脑浓度比血液浓度低约三分之一，而校正的体内递送预测的细胞外浓度与血液浓度非常相似。我们的结论是，乙醇的微透析校准方法需要一个测量的提取分数扩散到探针。需要进一步的研究来确定这种效应是否与其他醇类相同。
Traditional microdialysis techniques provide qualitative data, although quantitative data are often required for pharmacodynamic analyses. This study evaluated a potentially useful in vivo delivery technique to calibrate microdialysis probes for ethanol. We measured in vivo delivery extraction fractions within subjects across 2 days and found no change over time. We tested the effect of diffusion direction on extraction fraction and found that it was higher for ethanol diffusion out of the probe than for diffusion into the probe, both in vitro and in vivo, The in vivo extraction fraction ratio of diffusion,, Versus diffusion(OUT) was 0.65 +/- 0.03. Finally, we predicted extracellular brain ethanol concentrations after 1 g/kg ethanol administration using in vivo delivery, "no net flux" dialysis, or in vivo delivery corrected for diffusion direction with the in vivo extraction fraction ratio. Both in vivo delivery and "no net flux" dialysis predicted brain concentrations that were approximately one-third lower than blood concentrations, whereas the corrected in vivo delivery predicted extracellular concentrations very similar to blood concentrations. We conclude that microdialysis calibration methods for ethanol require a measure of extraction fraction for diffusion into the probe. Further studies are needed to establish whether this effect is common to other alcohols.