ANALYSIS OF FINE-STRUCTURE OF SURFACE-LAYERS FROM 2 STRAINS OF CLOSTRIDIA, INCLUDING CORRECTION FOR DISTORTED IMAGES
ANALYSIS OF FINE-STRUCTURE OF SURFACE-LAYERS FROM 2 STRAINS OF CLOSTRIDIA, INCLUDING CORRECTION FOR DISTORTED IMAGES
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DOI：
10.1016/s0022-5320(77)80006-3
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
SLEYTR, UB
中科院分区：
文献类型：
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作者：
CROWTHER, RA;SLEYTR, UB
用电子显微镜和计算机图像处理技术研究了热硫酸梭菌和热糖解梭菌细胞壁的表面层。这些层中的糖蛋白亚基形成规则的周期阵列，一个具有六方对称，另一个具有四方对称。由于晶格往往是弯曲的或以其他方式失真，开发了一种用于图像平均以降低噪声的计算机程序，该程序在平均之前考虑并校正空间失真。这样获得的平均图像显示的亚基的大小可以与糖蛋白的已知分子量相关联。亚基之间由细小的桥接物连接，为下面的细胞壁提供了良好的覆盖层。
EM and image processing by computer were used to study the surface layers from the cell walls of Clostridium thermohydrosulfuricum and C. thermosaccharolyticum. The glycoprotein subunits in these layers form regular periodic arrays, one with hexagonal symmetry, the other with tetragonal symmetry. Because the lattices tend to be curved or otherwise distorted, a computer procedure for image averaging to reduce noise was developed, which takes account of and corrects the spatial distortions prior to averaging. The average images so obtained show subunits of a size which can be correlated with the known molecular weight of the glycoprotein. The subunits are joined by fine bridges which provide a good covering of the underlying cell wall.