FIBRIN AND FIBRONECTIN IN RHEUMATOID SYNOVIAL-MEMBRANE AND RHEUMATOID SYNOVIAL-FLUID
FIBRIN AND FIBRONECTIN IN RHEUMATOID SYNOVIAL-MEMBRANE AND RHEUMATOID SYNOVIAL-FLUID
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DOI：
10.1002/art.1780260405
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
ANDERSEN, RB
中科院分区：
文献类型：
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作者：
CLEMMENSEN, I;HOLUND, B;ANDERSEN, RB
采用间接免疫过氧化物酶技术对典型类风湿关节炎患者的正常滑膜和滑膜进行纤维蛋白和纤维连接蛋白的检测。在正常滑膜中，在单层滑膜衬里细胞周围发现纤维连接蛋白。染色最强烈的是表面和下面的衬里细胞，但没有检测到细胞质。内皮细胞的细胞质中也有纤维连接蛋白。正常滑膜未见纤维蛋白染色。在类风湿性关节炎患者的滑膜中，在多层滑膜衬里细胞周围、内皮细胞的细胞质中以及亲阿糖纤维丰富的结缔组织中发现了大量的纤维连接蛋白。在滑膜衬里细胞脱落的浅表区域，纤维蛋白中掺入了大量的纤维连接蛋白。在一些滑膜衬里细胞未损伤的区域，也发现了纤维蛋白，但在这种情况下没有纤维连接蛋白的掺入。炎性细胞浸润区结缔组织未见纤维连接蛋白。在用透明质酸酶处理正常和类风湿滑膜后，纤维连接蛋白仍然存在于衬里细胞周围，但染色更加明显。这项研究将类风湿性滑膜中纤维蛋白和纤维连接蛋白的存在与最近在类风湿性滑膜液中描述的这些蛋白的高含量联系起来。滑膜中的纤维连接蛋白显然是由内皮细胞产生和分泌的。
Normal synovial membranes and synovial membranes from patients with classic rheumatoid arthritis were investigated for the presence of fibrin and fibronectin by an indirect immunoperoxidase technique. In normal synovial membranes, fibronectin was found around the monolayer of the synovial lining cells. Staining was most intense on the surface and beneath the lining cells, but not detectable in the cytoplasm. Fibronectin was also found in the cytoplasm of the endothelial cells. No staining for fibrin was found in the normal synovial membrane. In synovial membranes from patients with rheumatoid arthritis, large amounts of fibronectin were found around the multilayer of synovial lining cells, in the cytoplasm of the endothelial cells and in argyrophilic fiber-rich connective tissue. In superficial areas denuded of synovial lining cells, high amounts of fibronectin were found incorporated in fibrin. In some areas with noninjured synovial lining cells, fibrin was also found, but in this case no fibronectin was incorporated. No fibronectin was found in connective tissue in areas with infiltration of inflammatory cells. After treatment of normal and rheumatoid synovial membranes with hyaluronidase, fibronectin was still present around the lining cells but the staining was more distinct. This study relates the presence of fibrin and fibronectin in the rheumatoid synovial membrane to the high amount of these proteins, recently described, in rheumatoid synovial fluid. Fibronectin present in the synovial membrane apparently is produced and secreted by the endothelial cells.