OMICRON-QUINONES FORMED IN PLANT EXTRACTS - THEIR REACTIONS WITH AMINO ACIDS AND PEPTIDES
OMICRON-QUINONES FORMED IN PLANT EXTRACTS - THEIR REACTIONS WITH AMINO ACIDS AND PEPTIDES
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DOI：
10.1042/bj1120609
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发表时间：
1969-01-01
影响因子：
4.1
通讯作者：
PIERPOINT, WS
中科院分区：
文献类型：
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作者：
PIERPOINT, WS
1. 用压力法和分光光度法研究了绿原酸和咖啡酸酶氧化产生的氨基酸和多肽与茶醌的反应。2. 除赖氨酸和半胱氨酸外，氨基酸主要通过α-氨基反应生成红色或棕色产物。这些反应与醌的聚合相竞争，随后发生的二次反应可能会吸收氧气并产生其他颜色的产物。3. 赖氨酸的氨基以类似的方式与茶醌发生反应。半胱氨酸的巯基与醌发生反应，不吸收氧气，生成无色产物。4. 含有半胱氨酸的肽通过其巯基与茶醌反应。5. 其他肽，如甘氨酸-亮氨酸和亮氨酸-甘氨酸，主要通过它们的α-氨基进行反应，整个反应与α-末端氨基酸相似，只是速度更快。6. 对于某些肽，二级反应不同于发生在茶醌和末端氨基酸之间的反应。肌肽产生的颜色类似于组氨酸产生的颜色，而不是β-丙氨酸产生的颜色，而且脯氨酸与醌的反应比脯氨酸更复杂。
1. The reactions of amino acids and peptides with theo-quinones produced by the enzymic oxidation of chlorogenic acid and caffeic acid have been studied manometrically and spectrophotometrically. 2. Amino acids, except lysine and cysteine, react primarily through their α-amino groups to give red or brown products. These reactions, which compete with the polymerization of the quinones, are followed by secondary reactions that may absorb oxygen and give products with other colours. 3. The ∈-amino group of lysine reacts with theo-quinones in a similar way. The thiol group of cysteine reacts with the quinones, without absorbing oxygen, giving colourless products. 4. Peptides containing cysteine react with theo-quinones through their thiol group. 5. Other peptides, such as glycyl-leucine and leucylglycine, react primarily through their α-amino group and the overall reaction resembles that of theN-terminal amino acid except that it is quicker. 6. With some peptides, the secondary reactions differ from those that occur between theo-quinones and theN-terminal amino acids. The colours produced from carnosine resemble those produced from histidine rather than those from β-alanine, and the reactions of prolylalanine witho-quinones are more complex than those of proline.