Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose.
Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose.
复制标题
绕过戊糖磷酸盐途径：朝着木糖的模块化利用。
DOI：
10.1371/journal.pone.0158111
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Cate JH
中科院分区：
文献类型：
--
作者：
Chomvong K;Bauer S;Benjamin DI;Li X;Nomura DK;Cate JH
植物细胞壁中半纤维素的有效利用对于将植物生物质经济地转化为可再生燃料和化学品至关重要。先前，酵母酿酒酵母（Saccharomyces cerevisiae）已经被工程化以将半纤维素衍生的戊糖木糖和阿拉伯糖转化为d-木酮糖-5-磷酸，用于经由戊糖磷酸途径（PPP）转化。然而，有效的戊糖利用需要PPP优化，并可能干扰其在NADPH和戊糖生产中的作用。在这里，我们开发了一种替代木糖利用途径，在很大程度上绕过PPP。在新的途径中，d-木酮糖被转化为d-木酮糖-1-磷酸，这是S.酿酒酵母，其然后裂解为乙醇醛和二羟丙酮磷酸。该合成途径充当乙醇和乙二醇的生物合成的平台。使用d-木酮糖-1-磷酸作为木糖代谢的切入点为优化S.以模块化的方式构建酿酒酵母。
The efficient use of hemicellulose in the plant cell wall is critical for the economic conversion of plant biomass to renewable fuels and chemicals. Previously, the yeast Saccharomyces cerevisiae has been engineered to convert the hemicellulose-derived pentose sugars xylose and arabinose to d-xylulose-5-phosphate for conversion via the pentose phosphate pathway (PPP). However, efficient pentose utilization requires PPP optimization and may interfere with its roles in NADPH and pentose production. Here, we developed an alternative xylose utilization pathway that largely bypasses the PPP. In the new pathway, d-xylulose is converted to d-xylulose-1-phosphate, a novel metabolite to S. cerevisiae, which is then cleaved to glycolaldehyde and dihydroxyacetone phosphate. This synthetic pathway served as a platform for the biosynthesis of ethanol and ethylene glycol. The use of d-xylulose-1-phosphate as an entry point for xylose metabolism opens the way for optimizing chemical conversion of pentose sugars in S. cerevisiae in a modular fashion.