DISPERSED CELLS PREPARED FROM HUMAN PARATHYROID-GLANDS - DISTINCT CALCIUM SENSITIVITY OF ADENOMAS VS PRIMARY HYPERPLASIA
DISPERSED CELLS PREPARED FROM HUMAN PARATHYROID-GLANDS - DISTINCT CALCIUM SENSITIVITY OF ADENOMAS VS PRIMARY HYPERPLASIA
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DOI：
10.1210/jcem-46-2-267
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发表时间：
1978-01-01
影响因子：
5.8
通讯作者：
AURBACH, GD
中科院分区：
文献类型：
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作者：
BROWN, EM;BRENNAN, MF;AURBACH, GD
用胶原酶和DNA酶消化腺瘤或原发性增生患者的甲状旁腺，制备分散的甲状旁腺细胞。每克组织的产量为5000万-1亿个细胞，通过形态(95-100%台盼蓝排斥)和功能标准[甲状旁腺激素(PTH)对环境钙浓度的反应线性释放]，细胞是存活的。从9个增生性腺体中的7个和12个腺瘤性腺体中的8个制备的细胞可被钙抑制50%(52-90%)或以上。来自增生性腺体的细胞受到明显低于腺瘤细胞的钙的抑制，在这方面与正常的牛甲状旁腺细胞相似。这一结果提示，钙调节甲状旁腺激素释放的设定点误差可能与甲状旁腺腺瘤的病理生理有关。9个增生性腺体中的2个和12个腺瘤性腺体中的4个细胞在高钙浓度下可抑制50%(0-48%)，表明某些异常的甲状旁腺可能以真正的自主方式发挥作用。初步免疫学研究表明，分散细胞的孵育液可与N-端或C-端的抗血清反应，并与BPTH平行。无论是用N-或C-特异性测定，钙浓度升高都同样抑制分散细胞制剂中免疫反应性甲状旁腺素的释放。
Dispersed parathyroid cells were prepared by digestion with collagenase and DNase of parathyroid glands of patients with adenoma or primary hyperplasia. Yields of 50-100 million cells/g of tissue were obtained and the cells were viable by morphologic (95-100% trypan blue exclusion) and functional criteria [linear release of parathyroid hormone (PTH) responsive to ambient Ca concentration]. Cells prepared from 7 of 9 hyperplastic and 8 of 12 adenomatous glands were suppressible 50% (52-90%) or more by Ca. Cells from hyperplastic glands were suppressed by significantly lower concentrations of Ca than those from adenomas and resembled normal bovine [b] parathyroid cells in this respect. This results suggested that a set-point error in Ca-regulated PTH release might contribute to the pathophysiology of parathyroid adenomas. Cells from 2 of 9 hyperplastic and 4 of 12 adenomatous glands were suppressible < 50% (0-48%) by high Ca concentrations, indicating that some abnormal parathyroid glands may function in a truly autonomous fashion. Preliminary immunologic studies showed that incubation media from dispersed cells reacted with N- or C-terminal-directed antisera and parallel with bPTH. Elevated Ca concentrations suppressed immunoreactive PTH release from a preparation of dispersed cells similarly, whether measured by the N- or C-specific assay.