DIFFERENCES IN THE DEVELOPMENTAL FATE OF CULTURED AND NONCULTURED MYOBLASTS WHEN TRANSPLANTED INTO EMBRYONIC LIMBS
DIFFERENCES IN THE DEVELOPMENTAL FATE OF CULTURED AND NONCULTURED MYOBLASTS WHEN TRANSPLANTED INTO EMBRYONIC LIMBS
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DOI：
10.1006/excr.1995.1054
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发表时间：
1995-02-01
影响因子：
3.7
通讯作者：
STOCKDALE, FE
中科院分区：
文献类型：
--
作者：
DIMARIO, JX;STOCKDALE, FE
将来自胚胎、胎儿和成年鹌鹑和鸡肌肉的成肌细胞移植到鸡胚胎的肢芽中，以确定成肌细胞是否可以在异时肢中形成肌纤维，并确定影响移植细胞填充新发育的肢肌肉组织的能力的条件。将来自每个发育阶段的成肌细胞新鲜分离并移植或在移植到4至5天（ED 4 -5）鸡胚的肢芽中之前进行培养。移植的成肌细胞，无论其来源于供体的年龄如何，都在胚胎肢体肌肉内形成肌纤维。移植的克隆成肌细胞形成了肌纤维，尽管几乎没有证据表明移植的成肌细胞数量在移植后显著增加，或者它们从注射部位迁移了任何距离。从移植的克隆成肌细胞形成的纤维通常不持续在宿主肢体肌肉中，直到ED 10。无论成肌细胞是克隆的还是高密度培养的，移植到宿主肢体的成肌细胞纤维形成减少。然而，当移植新鲜分离的成肌细胞时，它们形成的纤维数量众多，广泛分散在肢体肌肉组织中，并持续存在于肌肉中，直到至少ED 10。这些结果表明，移植的胚胎，胎儿和成人来源的成肌细胞能够形成纤维在早期肢体肌肉形成。它们还表明，即使在内源性成肌细胞增殖和肌纤维形成快速进行的鸡胚胎肢体中，体外培养的成肌细胞在移植到发育肢体中后基本上不有助于长期肌纤维形成。然而，当相同的成肌细胞被新鲜分离并在没有预先细胞培养的情况下移植时，大量的纤维形成并在移植到发育中的肢体后持续存在。因此，这些研究表明，移植的成肌细胞融合形成在宿主肌肉组织中持续存在的纤维的程度取决于供体成肌细胞是新鲜分离的还是在注射前保持在体外。(C)出版社：Academic Press
Myoblasts from embryonic, fetal, and adult quail and chick muscles were transplanted into limb buds of chick embryos to determine if myoblasts can form muscle fibers in heterochronic limbs and to define the conditions that affect the ability of transplanted cells to populate newly developing limb musculature. Myoblasts from each developmental stage were either freshly isolated and transplanted or were cultured prior to transplantation into limb buds of 4- to 5-day (ED4-5) chick embryos. Transplanted myoblasts, regardless of the age of the donor from which they were derived, formed muscle fibers within embryonic limb muscles. Transplanted cloned myoblasts formed muscle fibers, although there was little evidence that the number of transplanted myoblasts significantly increased following transplantation or that they migrated any distance from the site of injection. The fibers that formed from transplanted clonal myoblasts often did not persist in the host limb muscles until ED10. Diminished fiber formation from myoblasts transplanted into host limbs was observed whether myoblasts were cloned or cultured at high density. However, when freshly isolated myoblasts were transplanted, the fibers they formed were numerous, widely dispersed within the limb musculature, and persisted in the muscles until at least ED10. These results indicate that transplanted myoblasts of embryonic, fetal, and adult origin are capable of forming fibers during early limb muscle formation. They also indicate that even in an embryonic chick limb where proliferation of endogenous myoblasts and muscle fiber formation is rapidly progressing, myoblasts that are cultured in vitro do not substantially contribute to long-term muscle fiber formation after they are transplanted into developing limbs. However, when the same myoblasts are freshly isolated and transplanted without prior cell culture, substantial numbers of fibers form and persist after transplantation into developing limbs. Thus, these studies demonstrate that the extent to which transplanted myoblasts fuse to form fibers which persist in host musculature depends upon whether donor myoblasts are freshly isolated or maintained in vitro prior to injection. (C) 1995 Academic Press, Inc.