Cloned Cattle Fetuses with the Same Nuclear Genetics Are More Variable Than Contemporary Half-Siblings Resulting from Artificial Insemination and Exhibit Fetal and Placental Growth Deregulation Even in the First Trimester1
Cloned Cattle Fetuses with the Same Nuclear Genetics Are More Variable Than Contemporary Half-Siblings Resulting from Artificial Insemination and Exhibit Fetal and Placental Growth Deregulation Even in the First Trimester1
复制标题
具有相同核遗传学的克隆牛胎儿比人工授精产生的当代同父异母兄弟姐妹的变异更大，即使在妊娠早期也表现出胎儿和胎盘生长失调1
DOI：
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发表时间：
2004
影响因子：
3.6
通讯作者：
David N. Wells
中科院分区：
文献类型：
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作者：
Rita S.F. Lee;A. James Peterson;Martyn J. Donnison;S. Ravelich;A. Ledgard;Ning Li;Jan E. Oliver;Andria L. Miller;F. Tucker;B. Breier;David N. Wells
摘要体细胞核移植克隆牛与胎盘形成异常、胎囊积液（水肿综合征）和胎儿过度生长的高发病率有关。在第50天（胎盘形成期间）、第100天（胎盘形成完成时）和第150天（水肿综合征频繁发生时）研究胎仔和胎盘发育。将NT胎儿与由体外产生的胚胎或人工授精（AI）产生的当代同父异母兄弟姐妹进行比较。胚胎子叶的形成和血管化的绒毛尿囊膜开始正常NT概念，但少数子叶成功地形成胎盘。到第100天，存活NT胎仔的胎盘平均数量显著较低。第150天，只有胎盘数量正常的孕妇才能存活。在第100天和第150天，存活NT组的胎盘体的平均总肉阜组织重量显著较高，与胎盘体数量无关，表明NT胎盘重量增加是由子宫组织过度生长引起的。到第100天，NT胎儿表现出生长失调，存活至第150天的胎儿比当代AI对照组重17%。第150天胎盘、肝脏和肾脏过度生长伴水肿综合征。NT胎儿过度生长不是体外胚胎培养的结果，也与胎盘过度生长无关。然而，体外培养和供体基因组的不完全重编程是表观遗传效应，可能会覆盖遗传性状，并导致NT组与AI半同胞相比胎盘和胎儿发育的变异性更大。
Abstract The cloning of cattle by somatic cell nuclear transfer (NT) is associated with a high incidence of abnormal placentation, excessive fluid accumulation in the fetal sacs (hydrops syndrome), and fetal overgrowth. Fetal and placental development was investigated at Day 50, during placentome formation; at Day 100, when placentation was completed; and at Day 150, when the hydrops syndrome frequently develops. The NT fetuses were compared with contemporary half-siblings generated from in vitro-produced embryos or by artificial insemination (AI). Fetal cotyledon formation and vascularization of the chorioallantoic membranes was initiated normally in NT conceptuses, but fewer cotyledons successfully formed placentomes. By Day 100, the mean number of placentomes was significantly lower in surviving NT fetuses. Only those with normal placentome numbers were represented in surviving NT pregnancies at Day 150. The mean total caruncle tissue weight of the placentomes was significantly higher in the surviving NT groups at Days 100 and 150, irrespective of the placentome numbers, indicating that increased NT placental weight was caused by excessive uterine tissue growth. By Day 100, NT fetuses exhibited growth deregulation, and those that survived to Day 150 were 17% heavier than contemporary AI controls. Placentome, liver, and kidney overgrowth accompanied the hydrops syndrome at Day 150. The NT fetal overgrowth was not a consequence of in vitro embryo culture and showed no correlation with placental overgrowth. However, in vitro culture and incomplete reprogramming of the donor genome are epigenetic effects that may override genetic traits and contribute to the greater variability in placental and fetal development in the NT group compared with AI half-siblings.
影响因子：
9.8
作者：
DeBaun, MR;Niemitz, EL;Feinberg, AP
通讯作者：
Feinberg, AP