Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence
Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence
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DOI：
10.1016/j.aca.2009.04.050
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发表时间：
2009-07-10
影响因子：
6.2
通讯作者：
Lin, Yuqing
中科院分区：
文献类型：
--
作者：
Li, Na;Guo, Jizhao;Lin, Yuqing
我们的前期工作表明，金纳米颗粒可以引发鲁米诺和硝酸银之间的化学发光（CL）。在本工作中，一些重要的生物还原性化合物，包括单胺类神经递质及其代谢产物，还原性氨基酸，抗坏血酸，尿酸，谷胱甘肽，对新的CL反应的影响进行了研究分析的目的。结果表明，它们均能抑制鲁米诺-AgNO_3-Au胶体体系的化学发光。其中，单胺类神经递质及其代谢产物表现出较强的抑制作用。以多巴胺为模型化合物，通过测定化学发光反应过程中的吸收光谱和停流法研究化学发光反应动力学，探讨了化学发光机理。其化学发光抑制机理可能是这些化合物与鲁米诺竞争AgNO 3，抑制鲁米诺自由基的形成，加速Ag原子在金纳米粒子表面的沉积，从而降低了化学发光强度。基于抑制的化学发光反应，建立了一种同时测定单胺类神经递质及其代谢产物的新方法。该方法已成功地应用于小鼠脑微透析液中化合物的测定。与文献报道的HPLC-CL方法相比，该方法具有简便、快速、可同时测定更多的分析物等优点。此外，使用所提出的方法的NE，E和DA的线性范围的限制比没有金纳米粒子的鲁米诺系统低一个数量级。(C)2009 Elsevier B. V.保留所有权利。
Our previous work showed that gold nanoparticles could trigger chemiluminescence (CL) between luminol and AgNO3. In the present work, the effect of some biologically important reductive compounds, including monoamine neurotransmitters and their metabolites, reductive amino acids, ascorbic acid, uric acid, and glutathione, on the novel CL reaction were investigated for analytical purpose. It was found that all of them could inhibit the CL from the luminol-AgNO3-Au colloid system. Among them, monoamine neurotransmitters and their metabolites exhibited strong inhibition effect. Taking dopamine as a model compound, the CL mechanism was studied by measuring absorption spectra during the CL reaction and the reaction kinetics via stopped-flow technique. The CL inhibition mechanism is proposed to be due to that these tested compounds competed with luminol for AgNO3 to inhibit the formation of luminol radicals and to accelerate deposition of Ag atoms on surface of gold nanoparticles, leading to a decrease in CL intensity. Based on the inhibited CL, a novel method for simultaneous determination of monoamine neurotransmitters and their metabolites was developed by coupling high-performance liquid chromatography with this CL reaction. The new method was successfully applied to determine the compounds in a mouse brain microdialysate. Compared with the reported HPLC-CL methods, the proposed method is simple, fast, and could determine more analytes. Moreover, the limits of linear ranges for NE, E, and DA using the proposed method were one order of magnitude lower than the luminol system without gold nanoparticles. (C) 2009 Elsevier B.V. All rights reserved.