Mesenchymal-epithelial interactions in bladder smooth muscle development: Epithelial specificity
Mesenchymal-epithelial interactions in bladder smooth muscle development: Epithelial specificity
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DOI：
10.1016/s0022-5347(01)62694-6
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发表时间：
1998-09-01
影响因子：
6.6
通讯作者：
Cunha, G
中科院分区：
文献类型：
--
作者：
DiSandro, MJ;Li, YW;Cunha, G
目的：我们先前的研究表明，间充质-上皮之间的相互作用对于膀胱平滑肌的发育是必要的。具体地说，没有膀胱上皮，胚胎膀胱间充质不能分化为平滑肌。我们确定这一过程是否是膀胱上皮所特有的，或者来自其他器官系统的上皮细胞是否诱导膀胱间充质向平滑肌分化，以及上皮年龄是否是一个重要的变量。材料和方法：将14天前的膀胱间充质与14天、19天的大鼠膀胱、输尿管、结肠、回肠、胃、角膜和表皮的上皮进行重组。此外，将膀胱上皮与14日龄胚胎小肠、14日龄胚胎胃和新生精囊间充质细胞进行重组。结果：免疫组织化学染色显示，所有上皮类型均可诱导膀胱间充质细胞分化为平滑肌细胞，但程度不同。平滑肌的诱导与尿路上皮年龄无关。此外，膀胱上皮可诱导肠、胃和精囊间充质细胞分化为平滑肌细胞，并表达膀胱纤维肌壁的整体形态。结论：尿路上皮细胞诱导膀胱间充质细胞分化为平滑肌的机制不是胚胎尿路上皮所特有的。较老的尿路上皮和异型上皮也能诱导平滑肌分化。随着肠、胃和输尿管段膀胱扩大术的广泛使用，了解不同类型的上皮与天然膀胱的相互作用是很重要的。
Purpose: We previously showed that mesenchymal-epithelial interactions are necessary for the development of bladder smooth muscle. Specifically without bladder epithelium embryonic bladder mesenchyme does not differentiate into smooth muscle. We determine whether this process is specific to bladder epithelium or whether epithelial cells from other organ systems induce bladder mesenchyme to differentiate into smooth muscle, as well as whether epithelial age is an important variable.Materials and Methods: We recombined 14-day bladder mesenchyme before smooth muscle differentiation with rat epithelium from 14-day, 19-day, newborn and adult bladder, ureter, colon, ileum, stomach, cornea and epidermis. In addition, bladder epithelium was recombined with 14-day embryonic small intestinal, 14-day embryonic gastric and newborn seminal vesicle mesenchyme. All tissue recombinants were grafted under the renal capsule of an adult rat syngeneic host for 3 weeks.Results: Immunohistochemical analysis with antibodies directed against smooth muscle alpha-actin revealed that all epithelial types studied induced bladder mesenchyme to differentiate into smooth muscle, although to different degrees. Induction of smooth muscle was independent of urothelial age. In addition, bladder epithelium induced intestinal, gastric and seminal vesicle mesenchyme to differentiate into smooth muscle and express an overall morphological pattern indicative of the bladder fibromuscular wall.Conclusions: The mechanism whereby urothelium induces bladder mesenchyme to differentiate into smooth muscle is not specific to embryonic urothelium. Older urothelium and heterotypic epithelium also induce smooth muscle differentiation. With the common use of bowel, stomach and ureteral segments for bladder augmentation it is important to understand the interaction of different types of epithelium with the native bladder.