Settings, Quantification, and Statistical Analyses beyond p-Values for Arabidopsis Ethylene Responses
Settings, Quantification, and Statistical Analyses beyond p-Values for Arabidopsis Ethylene Responses
复制标题
拟南芥乙烯反应超出 p 值的设置、定量和统计分析
DOI：
10.1002/smtd.201900386
复制
发表时间：
2019-08-13
期刊：
影响因子：
12.4
通讯作者：
Wen, Chi-Kuang
中科院分区：
文献类型：
--
作者：
Lu, Jian;Wen, Chi-Kuang
乙烯是一种气体植物激素，在植物生长发育中起着多种作用。乙烯诱导的响应的研究已经推进了乙烯信号及其与其他植物激素和生物和非生物线索的相互作用的影响的知识。乙烯响应的程度可以基于“幼苗三重响应测定”来定量，所述测定主要测量黄化的拟南芥幼苗的下胚轴和/或根长度。不同的实验室进行不同的测定，可能具有不同程度的任意性，并且独立研究的实验结果很难共享和比较。一个最佳的和标准化的协议，为乙烯处理的设置可能会促进数据共享，复制，和新的发现。从实验中收集的数据进行量化和统计分析，以支持科学推断。另一方面，错误的统计实践被批评为混淆科学推断，导致重复性差和错误的结果。掌握统计学的基本概念可以避免错误的做法和推论。在这项研究中，乙烯处理预处理的不同设置在现场进行了比较，统计分析，以解释如何设置可能会影响结果，并提出了一个标准化的进行幼苗三重反应测定。
Ethylene is a gaseous plant hormone and plays various roles in plant growth and development. Studies on ethylene-induced responses have advanced the knowledge about ethylene signaling and effects of its interactions with other plant hormones and with biotic and abiotic cues. Degrees of the ethylene response can be quantified on the basis of the "seedling triple-response assay" that primarily measures the hypocotyl and/or root lengths of etiolated Arabidopsis seedlings. Different laboratories perform the assay differently, possibly with various degrees of arbitrariness, and experimental results across independent studies can hardly be shared and compared. An optimal and standardized protocol for the setting of ethylene treatment may facilitate data sharing, reproducing, and new findings. Data collected from experiments are quantified and analyzed statistically to support scientific inference. On the other hand, erroneous statistical practices are criticized for muddling scientific inference, leading to poor reproducibility and false results. Grasping the basic concepts of statistics may avoid erroneous practices and inference. Different settings for ethylene treatment prevalently adapted in the field are compared in this study, analyzed statistically to explain how the settings may affect the outcome, and a standardized conduct for the seedling triple-response assay is proposed.