Fate mapping the avian neural plate with quail/chick chimeras: origin of prospective median wedge cells.
Fate mapping the avian neural plate with quail/chick chimeras: origin of prospective median wedge cells.
复制标题
用鹌鹑/小鸡嵌合体绘制鸟类神经板的命运：预期中位楔形细胞的起源。
DOI：
10.1002/jez.1402490305
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Vakaet,L
中科院分区：
文献类型：
--
作者：
Schoenwolf,GC;Bortier,H;Vakaet,L
未来的M细胞是正中神经上皮细胞，在神经板弯曲过程中变得楔形，最终形成神经管的中线底板，通过构建鹌鹑/鸡嵌合体并使用鹌鹑核仁标记识别鸡囊胚层中的鹌鹑供体细胞来确定预期M细胞的来源。研究了上胚层中两个可能的M细胞起源部位：位于Hensen‘s结头正上方的单个中线雏形和原始条纹颅部两侧的成对雏形。我们的结果表明，M细胞只起源于中线，结前的雏形。M细胞从这个雏形向尾部延伸，贯穿神经上皮的整个长度。这些关于未来M细胞起源的新信息将有助于分析它们在神经形成中的作用。
The origin of prospective M cells, which are median neuroepithelial cells that become wedge‐shaped during bending of the neural plate and eventually form the midline floor of the neural tube, was determined by constructing quail/chick chimeras and using the quail nucleolar marker to identify quail donor cells in chick host blastoderms. Two possible sites of prospective M‐cell origin in the epiblast were examined: a single, midline rudiment located just rostral to Hensen's node and paired rudiments flanking the cranial part of the primitive streak. Our results suggest that M cells arise exclusively from the midline, prenodal rudiment. From this rudiment, M cells extend caudally throughout the entire length of the neuroepithelium. This new information on the origin of prospective M cells will aid in the analysis of their role in neurulation.