Characterization of Treponema denticola pyrF encoding orotidine-5'-monophosphate decarboxylase.
Characterization of Treponema denticola pyrF encoding orotidine-5'-monophosphate decarboxylase.
复制标题
编码乳清苷-5-单磷酸脱羧酶的密螺旋体pyrF的表征。
DOI：
10.1111/j.1574-6968.2006.00589.x
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发表时间：
2007
影响因子：
2.1
通讯作者：
ChristopherFenno,J
中科院分区：
文献类型：
--
作者：
Capone,RicardoF;Ning,Yu;Pakulis,Nora;Alhazzazi,Turki;ChristopherFenno,J
TheTreponema denticolaATCC 35405 genome annotation contains most of the genes forde novopyrimidine biosynthesis. To initiate characterization of pyrimidine synthesis inTreponema, we focused on TDE2110 (the putativepyrF, encoding orotidine-5′-monophosphate decarboxlyase). Unlike the parent strain, an isogenicpyrFmutant was resistant to 5-fluoroorotic acid. In complex medium, growth of thepyrFmutant was independent of added uracil, indicating activity of a uracil uptake/salvage pathway. Transcription ofpyrFwas greatly reduced inT. denticolagrown in excess uracil, demonstrating thatde novopyrimidine synthesis is regulated and suggesting a feedback mechanism.Treponema denticolaPyrF complemented uracil auxotrophy in anEscherichia coli pyrFmutant. This study provides biochemical confirmation ofT. denticolagenome predictions ofde novoand salvage pyrimidine pathways and provides proof of concept thatpyrFhas potential as a selectable marker inT. denticola.
