Bradyrhizobium ottawaense efficiently reduces nitrous oxide through high nosZ gene expression.
Bradyrhizobium ottawaense efficiently reduces nitrous oxide through high nosZ gene expression.
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DOI：
10.1038/s41598-023-46019-w
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发表时间：
2023-11-01
影响因子：
4.6
通讯作者：
Minamisawa, Kiwamu
中科院分区：
文献类型：
--
作者：
Wasai-Hara, Sawa;Itakura, Manabu;Siqueira, Arthur Fernandes;Takemoto, Daisaku;Sugawara, Masayuki;Mitsui, Hisayuki;Sato, Shusei;Inagaki, Noritoshi;Yamazaki, Toshimasa;Imaizumi-Anraku, Haruko;Shimoda, Yoshikazu;Minamisawa, Kiwamu
N2O 是影响全球变暖的重要温室气体，农业用地是 N2O 排放的主要（人为）来源。在这里，我们报告了渥太华慢生根瘤菌的高 N2O 还原活性，表明其具有有效减少农田 N2O 排放的潜力。在检查的 15 个 B. ottawaense 分离株中，大多数（13 个）菌株的 N2O 还原活性比厌氧条件下的重氮慢生根瘤菌 USDA110T 高约五倍。 B. ottawaense 的这种强大的 N2O 还原活性通过 N2O 还原酶 (NosZ) 蛋白水平和实验室系统中根瘤分解排放的 N2O 的减少得到了证实。虽然B. ottawaense和B. diazoefficiens的NosZ表现出高度同源性，但B. ottawaense中的nosZ基因表达量比B. diazoefficiens USDA110T高150倍以上，表明B. ottawaense的高N2O还原活性是通过高nos表达实现的。此外，我们检查了nos操纵子转录起始位点，发现与B. diazoefficiens不同，B. ottawaense在N2O呼吸条件下有两个转录起始位点，这可能有助于nosZ的高表达。我们的研究表明 B. ottawaense 具有有效减少 N2O 和对 nos 基因表达进行独特调节以实现土壤中 N2O 高效缓解的潜力。
N2O is an important greenhouse gas influencing global warming, and agricultural land is the predominant (anthropogenic) source of N2O emissions. Here, we report the high N2O-reducing activity of Bradyrhizobium ottawaense, suggesting the potential for efficiently mitigating N2O emission from agricultural lands. Among the 15 B. ottawaense isolates examined, the N2O-reducing activities of most (13) strains were approximately five-fold higher than that of Bradyrhizobium diazoefficiens USDA110T under anaerobic conditions. This robust N2O-reducing activity of B. ottawaense was confirmed by N2O reductase (NosZ) protein levels and by mitigation of N2O emitted by nodule decomposition in laboratory system. While the NosZ of B. ottawaense and B. diazoefficiens showed high homology, nosZ gene expression in B. ottawaense was over 150-fold higher than that in B. diazoefficiens USDA110T, suggesting the high N2O-reducing activity of B. ottawaense is achieved by high nos expression. Furthermore, we examined the nos operon transcription start sites and found that, unlike B. diazoefficiens, B. ottawaense has two transcription start sites under N2O-respiring conditions, which may contribute to the high nosZ expression. Our study indicates the potential of B. ottawaense for effective N2O reduction and unique regulation of nos gene expression towards the high performance of N2O mitigation in the soil.
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影响因子：
3.6
作者：
Henault, Catherine;Barbier, Elodie;Revellin, Cecile
通讯作者：
Revellin, Cecile
影响因子：
4.4
作者：
Itakura, Manabu;Tabata, Kazufumi;Minamisawa, Kiwamu
通讯作者：
Minamisawa, Kiwamu
影响因子：
5.2
作者：
Torres MJ;Bueno E;Jiménez-Leiva A;Cabrera JJ;Bedmar EJ;Mesa S;Delgado MJ
通讯作者：
Delgado MJ
影响因子：
4.8
作者：
Bonnet, Mariette;Kurz, Mareike;Gruetter, Markus G.
通讯作者：
Gruetter, Markus G.
影响因子：
5.2
作者：
Bueno E;Mania D;Frostegard Ǻ;Bedmar EJ;Bakken LR;Delgado MJ
通讯作者：
Delgado MJ