YEAST GLYCOPROTEIN-BIOSYNTHESIS - MNT1 ENCODES AN ALPHA-1,2-MANNOSYLTRANSFERASE INVOLVED IN O-GLYCOSYLATION
YEAST GLYCOPROTEIN-BIOSYNTHESIS - MNT1 ENCODES AN ALPHA-1,2-MANNOSYLTRANSFERASE INVOLVED IN O-GLYCOSYLATION
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DOI：
10.1073/pnas.89.15.6846
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发表时间：
1992-08-01
影响因子：
11.1
通讯作者：
ROBBINS, PW
中科院分区：
文献类型：
--
作者：
HAUSLER, A;BALLOU, L;ROBBINS, PW
酿酒酵母MNT 1基因编码高尔基甘露糖基转移酶。MNT 1基因座的基因破坏导致α-甲基甘露糖苷作为受体的特异性α-1，2-甘露糖基转移酶活性降低>90%。MNT 1的突变体是活的，没有明显的生长缺陷，并且在蛋白质O-连接的甘露二糖的延伸中被阻断。从mnn 1 mnn 10 mnt 1菌株分离的N-连接外链的结构分析显示，与亲本mnn 1 mnn 10菌株相比，碳水化合物结构没有改变。MNT 1基因与KRE 2相同，基因突变使细胞对沙门氏菌的杀伤毒素K1具有抗性。这表明O-甘露糖基化蛋白在抗性机制中的作用。此外，MNT 1是一个多基因家族的一部分，其成员被认为是酵母高尔基甘露糖基转移酶。
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a >90% reduction of specific alpha-1,2-mannosyltransferase activity with alpha-methylmannoside as acceptor. Null mutants of MNT1 are viable, have no apparent growth defect, and are blocked in the elongation of protein O-linked mannobiose. Structural analysis of the N-linked outer chain isolated from an mnn1 mnn10 mnt1 strain revealed no alteration in carbohydrate structure compared to the parental mnn1 mnn10 strain. The MNT1 gene is identical to KRE2, and mutations in the gene render cells resistant to the killer toxin K1 of S. cerevisiae, which suggests a role for O-mannosylated proteins in the resistance mechanism. In addition, MNT1 is part of a multigene family whose members are presumed to be yeast Golgi mannosyltransferases.