Mechanism of thyroid calorigenesis: role of active sodium transport.
Mechanism of thyroid calorigenesis: role of active sodium transport.
复制标题
甲状腺产热机制：主动钠转运的作用。
DOI：
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发表时间：
1970
影响因子：
11.1
通讯作者：
I. Edelman
中科院分区：
文献类型：
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作者：
F. Ismail;I. Edelman
通过测量甲状腺素和三碘甲腺原氨酸注射的甲状腺切除大鼠和正常大鼠的肝切片和骨骼肌（隔膜）的 Q（o2），在用哇巴因（10（-3）M）和/或不含 Na（+）或 K（+）强化的培养基中测试甲状腺热量生成是通过刺激主动 Na（+）转运介导的假设。在这两种组织中，甲状腺功能正常的大鼠注射甲状腺激素产生的 Q(o2) 增加超过 90% 来自 Na(+) 泵能量利用率的增加。在三碘甲状腺原氨酸治疗的甲状腺切除大鼠中，Na(+)转运的激活占肝脏中Q(o2)增量的90%或更多，以及膈肌中Q(o2)增量的40%或更多。在甲状腺功能正常和甲状腺功能亢进的肝脏和膈肌中测量细胞内Na(+)、K(+)和Cl(-)浓度。给予三碘甲状腺原氨酸后，两种组织中跨膜Na(+)和K(+)浓度差异均显着增加。这些结果表明，甲状腺激素通过增加局部ATP浓度或直接刺激Na(+)泵来激活Na(+)排出和K(+)积累。
The hypothesis that thyroid calorigenesis is mediated by stimulation of active Na(+) transport was tested by measuring the Q(o2) of liver slices and skeletal muscle (diaphragm) from thyroxine- and triiodothyronine-injected thyroidectomized and normal rats in media fortified with ouabain (10(-3) M) and/or free of Na(+) or K(+). In both tissues, more than 90% of the increase in Q(o2) produced by injections of thyroid hormone in euthyroid rats was derived from increased energy utilization by the Na(+) pump. In triiodothyronine-treated thyroidectomized rats, activation of Na(+) transport accounted for 90% or more of the increment in Q(o2) in liver and 40% or more of the increment in diaphragm. Intracellular Na(+), K(+), and Cl(-) concentrations were measured in euthyroid and hyperthyroid liver and diaphragm. The transmembrane Na(+) and K(+) concentration differences were significantly increased in both tissues by the administration of triiodothyronine. These results indicate that thyroid hormone activates Na(+) extrusion and K(+) accumulation either by increasing the local concentration of ATP or by direct stimulation of the Na(+) pump.