Melting behaviour of schizophyllan extracellular polysaccharide gels in the temperature range between 5 and 20°C
Melting behaviour of schizophyllan extracellular polysaccharide gels in the temperature range between 5 and 20°C
复制标题
裂褶多糖胞外多糖凝胶在5~20°C温度范围内的熔化行为
DOI：
10.1016/s0144-8617(00)00270-8
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发表时间：
2001
影响因子：
11.2
通讯作者：
A. Bot
中科院分区：
文献类型：
--
作者：
A. Bot
发现由具有1，6-β-d-葡糖基侧基的葡萄糖残基的1，3-β-d-连接的主链组成的葡聚糖葡聚糖的凝胶在5 ° C和20°C之间的温度范围内显示出熔融行为，这取决于溶剂中的葡萄糖浓度（0- 50重量%葡萄糖）。虽然凝胶的模量-浓度和模量-温度流变学数据的定性特征可以使用改进的级联理论（其隐含地假设凝胶结构中不存在组织的子水平）来建模，但是不能实现一致的定量拟合。所发现的不一致性与以下想法一致：凝胶由束（由许多三重螺旋组成）组成，具有强的束内吸引力和弱的束间力。稀释样品的透射电子显微镜（TEM）显微照片表明，在低于熔融温度的温度下，珠蛋白聚合物参与三螺旋链的侧向聚集，表明聚合物束确实将以凝胶状态存在。
Gels of the glucan schizophyllan, consisting of a 1,3-β-d-linked backbone of glucose residues with 1,6-β-d-glucosyl side groups, were found to show melting behaviour in the temperature range between 5 and 20°C, depending on the glucose concentration in the solvent (0–50wt% glucose). While the qualitative features of the modulus-versus-concentration and modulus-versus-temperature rheological data for the gels can be modelled using modified cascade theory (which implicitly assumes that no sub-level of organisation exists in the gel structure), a consistent quantitative fit cannot be achieved. The inconsistencies found are consistent with the idea that the gel is composed of bundles (consisting of many triple helices of schizophyllan) with strong intra-bundle attraction and weak inter-bundle forces. Transmission Electron Microscopy (TEM) micrographs of diluted samples indicate that schizophyllan polymers engage in lateral aggregation of triple helical strands at temperatures below the melting temperature, suggesting that indeed bundles of polymers will be present in the gel state.