Next-generation in vivo optical imaging with short-wave infrared quantum dots
Next-generation in vivo optical imaging with short-wave infrared quantum dots
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DOI：
10.1038/s41551-017-0056
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发表时间：
2017-04-01
影响因子：
28.1
通讯作者：
Bawendi, Moungi G.
中科院分区：
文献类型：
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作者：
Bruns, Oliver T.;Bischof, Thomas S.;Bawendi, Moungi G.
对于体内成像，短波长红外区域（SWIR; 1，000 - 2，000 nm）提供了优于可见光和近红外区域的几个优点：通常缺乏自发荧光，血液和组织的低光吸收，以及减少的散射。然而，缺乏通用的和功能的短波红外发射器，阻止了生物医学研究界普遍采用短波红外成像。在这里，我们介绍了一类高品质的SWIR发射砷化铟为基础的量子点，很容易修改的各种功能成像应用，并表现出窄和尺寸可调的发射和显着更高的发射量子产率比以前描述的SWIR探针。为了证明SWIR量子点所提供的前所未有的深穿透、高空间分辨率、多色成像和快速采集速度的组合，我们在小鼠中同时和在真实的时间内量化了几个器官中脂蛋白的代谢周转率以及清醒和不受约束的动物的心跳和呼吸率，并生成了小鼠脑血管系统的详细三维定量流图。
For in vivo imaging, the short-wavelength infrared region (SWIR; 1,000-2,000 nm) provides several advantages over the visible and near-infrared regions: general lack of autofluorescence, low light absorption by blood and tissue, and reduced scattering. However, the lack of versatile and functional SWIR emitters has prevented the general adoption of SWIR imaging by the biomedical research community. Here, we introduce a class of high-quality SWIR-emissive indium-arsenide-based quantum dots that are readily modifiable for various functional imaging applications, and that exhibit narrow and size-tunable emission and a dramatically higher emission quantum yield than previously described SWIR probes. To demonstrate the unprecedented combination of deep penetration, high spatial resolution, multicolour imaging and fast acquisition speed afforded by the SWIR quantum dots, we quantified, in mice, the metabolic turnover rates of lipoproteins in several organs simultaneously and in real time as well as heartbeat and breathing rates in awake and unrestrained animals, and generated detailed three-dimensional quantitative flow maps of the mouse brain vasculature.