Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia
Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia
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DOI：
10.1086/514127
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发表时间：
1997-11-01
影响因子：
6.4
通讯作者：
Warren, HS
中科院分区：
文献类型：
--
作者：
Ge, YM;Ezzell, RM;Warren, HS
研究了静脉注射纯化的 LPS 或活大肠杆菌后大鼠组织中免疫反应性白细胞介素 -1 (IL-1) 和脂多糖 (LPS) 的分布。脾脏中的 IL-1 染色在 4-8 小时达到峰值，与边缘区巨噬细胞中的 LPS 共定位，注射后 24 小时检测不到，而 LPS 染色在 24 小时达到峰值，并在 4 周内可检测到。LPS 和活细菌的组织 IL-1 反应相似。因此，尽管LPS在相同细胞中维持数周，但组织IL-1在数小时内下调；尽管LPS染色强烈，肝脏和肺中的巨噬细胞仅具有轻微的IL-1染色。革兰氏阴性菌血症后，组织 IL-1 的产生似乎受到差异性调节；肝脏和肺巨噬细胞清除的 LPS 会产生最少的 IL-1，而脾脏边缘区局部产生大量 IL-1，可能会增加对细菌壁抗原的免疫反应。
Distributions of immunoreactive interleukin-l (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL-1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks, The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is downregulated within hours despite maintenance of LPS in the same cells for weeks, Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.