LcSAIN1, a Novel Salt-Induced Gene from SheepGrass, Confers Salt Stress Tolerance in Transgenic Arabidopsis and Rice
LcSAIN1, a Novel Salt-Induced Gene from SheepGrass, Confers Salt Stress Tolerance in Transgenic Arabidopsis and Rice
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LcSAIN1，一种来自羊草的新型盐诱导基因，赋予转基因拟南芥和水稻耐盐胁迫的能力
DOI：
10.1093/pcp/pct069
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发表时间：
2013-07-01
影响因子：
4.9
通讯作者：
Liu, Gongshe
中科院分区：
文献类型：
--
作者：
Li, Xiaoxia;Hou, Shenglin;Liu, Gongshe
之前，我们通过下一代测序比较了绵羊草（羊草，禾本科：小麦科）中高盐胁迫诱导的> 1，500个基因在响应高盐胁迫时的转录水平变化。在已鉴定的基因中，一个功能未知的基因（命名为Leymus chinensis salt-induced 1，LcSAIN 1）与小麦、大麦和水稻中的同源基因序列高度一致，但LcSAIN 1及其同源基因产生的蛋白质不具有保守的功能结构域。LcSAIN 1基因的转录受到各种胁迫的上调。与对照植物相比，LcSAIN 1在拟南芥和水稻中的过表达增加了子叶的绿化率、鲜重、根伸长、株高和植物存活率，并赋予了对盐胁迫的耐受性。亚细胞定位分析表明LcSAIN 1主要定位于细胞核。我们的研究结果表明，LcSAIN 1基因可能在盐胁迫下增加转基因植物中转录因子（MYB 2和DREB 2A）和功能基因（P5 CS和RAB 18）的表达方面发挥重要的正向调节作用，它通过积累相容性溶质（脯氨酸和可溶性糖）和缓解活性氧的变化来增强胁迫耐受性。LcSAIN 1基因可能成为重要作物耐盐性工程的潜在资源。
Previously, we identified > 1,500 genes that were induced by high salt stress in sheepgrass (Leymus chinensis, Gramineae: Triticeae) when comparing the changes in their transcription levels in response to high salt stress by next-generation sequencing. Among the identified genes, a gene of unknown function (designated as Leymus chinensis salt-induced 1, LcSAIN1) showed a high sequence identity to its homologs from wheat, Hordeum vulgare and Oryza sativa, but LcSAIN1 and its homologs produce hypothetical proteins with no conserved functional domains. Transcription of the LcSAIN1 gene was up-regulated by various stresses. The overexpression of LcSAIN1 in Arabidopsis and rice increased the greening rate of cotyledons, the fresh weight, root elongation, plant height and the plant survival rate when compared with control plants and conferred a tolerance against salt stress. Subcellular localization analysis indicated that LcSAIN1 is localized predominantly in the nucleus. Our results show that the LcSAIN1 gene might play an important positive modulation role in increasing the expression of transcription factors (MYB2 and DREB2A) and functional genes (P5CS and RAB18) in transgenic plants under salt stress and that it augments stress tolerance through the accumulation of compatible solutes (proline and soluble sugar) and the alleviation of changes in reactive oxygen species. The LcSAIN1 gene could be a potential resource for engineering salinity tolerance in important crop species.