Cell sheets using human amniotic fluid stem cells reduce tissue fibrosis in murine full-thickness skin wounds
Cell sheets using human amniotic fluid stem cells reduce tissue fibrosis in murine full-thickness skin wounds
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DOI：
10.1016/j.tice.2020.101472
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发表时间：
2021-02-01
期刊：
影响因子：
2.6
通讯作者：
Tanaka, Mamoru
中科院分区：
文献类型：
--
作者：
Ochiai, Daigo;Abe, Yushi;Tanaka, Mamoru
使用间充质干细胞片是一种很有前途的皮肤再生策略。最近发现，注射分离的人羊水干细胞（hAFSCs）可以加速皮肤伤口愈合，减少纤维化瘢痕，类似于胎儿伤口愈合。然而，hAFSCs在细胞片技术中的应用仍然有限。本研究的目的是确定体外培养的hAFSC片在伤口愈合中的体内效果。采用免疫组织化学和RT-qPCR方法对细胞进行鉴定，并将其移植到BALB/c小鼠全层创面上。测量创面大小，对再生创面的再上皮化、肉芽组织面积、胶原蛋白含量进行组织学分析。虽然hAFSC片含有丰富的细胞外基质分子并表达高水平的抗纤维化介质，但其移植不影响伤口愈合或肉芽组织面积的大小。相比之下，hAFSC片植入后，再生创面中I型胶原束的组织明显减少，而III型胶原束的水平则增加。这些结果表明，hAFSC片可以发挥抗纤维化特性，而不会延迟伤口愈合。
The use of mesenchymal stem cell sheets is a promising strategy for skin regeneration. The injection of dissociated human amniotic fluid stem cells (hAFSCs) was recently found to accelerate cutaneous wound healing with reduced fibrotic scarring, similar to fetal wound healing. However, the use of hAFSCs in applications of cell sheet technology remains limited. The aim of this study was to determine the in vivo efficacy of in vitro-cultured hAFSC sheets in wound healing. The cell sheets were characterized by immunohistochemistry and RT-qPCR and grafted onto full-thickness wounds in BALB/c mice. The wound size was measured, and re-epithelialization, granulation tissue area, and collagen content of the regenerated wound were analyzed histologically. Although the hAFSC sheet contained abundant extracellular matrix molecules and expressed high levels of anti-fibrotic mediators, its grafting did not affect wound closure or the size of the granulation tissue area. In contrast, the organization of type I collagen bundles in the regenerated wound was markedly reduced, while the levels of type III collagen were increased after implantation of the hAFSC sheet. These results suggest that hAFSC sheets can exert antifibrotic properties without delaying wound closure.