ESTROGEN AND PROGESTERONE RECEPTORS IN SCREEN-DETECTED BREAST-CARCINOMA - AN IMMUNOHISTOLOGICAL STUDY USING PARAFFIN SECTIONS
ESTROGEN AND PROGESTERONE RECEPTORS IN SCREEN-DETECTED BREAST-CARCINOMA - AN IMMUNOHISTOLOGICAL STUDY USING PARAFFIN SECTIONS
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DOI：
10.1111/j.1365-2559.1992.tb00442.x
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发表时间：
1992-12-01
期刊：
影响因子：
6.4
通讯作者：
SINNETT, HD
中科院分区：
文献类型：
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作者：
SOOMRO, S;SHOUSHA, S;SINNETT, HD
使用单克隆抗体ER-ICA和PgR-ICA（Abbott）和免疫过氧化物酶技术，在81例筛查检测的乳腺癌石蜡切片中研究了雌激素和孕激素受体的存在。将免疫组织学结果与28个肿瘤中标准葡聚糖包被的活性炭生化测定的结果进行比较，这些肿瘤足够大以提供用于该测定的肿瘤组织。雌激素受体阳性63例（78%），孕激素受体阳性62例（77%）。在可触及或不可触及、原位或浸润性肿瘤中受体阳性率之间无统计学差异。在28例进行了生化检测的病例中，两种方法分别在23例（82%）和21例（75%）肿瘤中获得了相似的雌激素和孕激素受体结果。两种受体的平均生化数值和半定量组织学评分之间也存在良好的相关性。它的结论是，评估受体状态的小屏幕检测癌是可行的，使用常规处理的石蜡切片。有合理的良好的相关性，通过标准的葡聚糖包被的活性炭生物化学测定法获得的结果，但更多的真正的受体阳性的情况下，通过免疫组织学检测。
The presence of oestrogen and progesterone receptors was studied in paraffin sections of 81 screen-detected breast carcinomas using the monoclonal antibodies ER-ICA and PgR-ICA (Abbott) and the immunoperoxidase technique. The immunohistological results were compared with the results of the standard dextran-coated charcoal biochemical assay in 28 tumours which were big enough to provide tumour tissue for this assay. Sixty-three cases (78%) were oestrogen receptor positive and 62 (77%) were progesterone receptor positive. There was no statistical difference between receptor positivity in palpable or impalpable, in situ or invasive tumours. In the 28 cases where the biochemical assay was carried out, the two methods gave similar results in 23 (82%) and 21 (75%) tumours for oestrogen and progesterone receptors respectively, The majority of the remaining tumours, with one exception, were positive with immunohistology and negative with biochemistry. A good correlation was also present between the mean numerical biochemical values and the semiquantitative histological scores for both receptors. It is concluded that assessment of receptor status of small screen-detected carcinomas is feasible using routinely processed paraffin sections. There is reasonably good correlation with the results obtained by the standard dextran-coated charcoal biochemical assay, but more genuine receptor positive cases are detected by immunohistology.