QUANTITATIVE-ANALYSIS OF CELL TURNOVER IN ENAMEL ORGAN OF RAT INCISOR - EVIDENCE FOR AMELOBLAST DEATH IMMEDIATELY AFTER ENAMEL MATRIX SECRETION
QUANTITATIVE-ANALYSIS OF CELL TURNOVER IN ENAMEL ORGAN OF RAT INCISOR - EVIDENCE FOR AMELOBLAST DEATH IMMEDIATELY AFTER ENAMEL MATRIX SECRETION
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DOI：
10.1002/ar.1091870106
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发表时间：
1977-01-01
影响因子：
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通讯作者：
WARSHAWSKY, H
中科院分区：
文献类型：
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作者：
SMITH, CE;WARSHAWSKY, H
在大鼠切牙的釉质器官更新过程中，上皮细胞群依次通过分泌前区、分泌区和成熟区运输到牙龈缘，在那里这些细胞的生命周期终止。这个过程进行了检查动力学确定的绝对流量的细胞内的每个区域的釉质。分泌前区的成釉细胞、中间层和乳头层细胞的流出量约等于分泌区的流出量加上预期的生长量。然而，在分泌区和成熟区之间，约50%的成釉细胞进入成熟区，而不是考虑到牙龈缘的出口和预期的生长。由于乳头层细胞在这些区域之间没有类似的不平衡，因此得出结论，这种差异必须代表在成釉细胞成熟阶段成釉细胞群体的大小减少50%。略高于25%的损失发生在开始成熟的分泌后过渡的区域内，其余的25%的损失发生在整个后续地区的成熟区。除了动力学分析之外，还准备了成釉细胞核的图形重建或表面图。这些图谱说明了成釉细胞核在成釉作用的3个区域内堆积的特征，并且它们提供了定量证实，随着成釉细胞通过成熟区的进展，存在通过动力学分析预测的量的细胞损失。
During renewal of the enamel organ in the rat incisor cohorts of epithelial cells are transported sequentially through presecretory, secretory and maturation zones to the gingival margin where the life cycles of these cells terminate. This process was examined kinetically by determining the absolute flux of cells within each of these zones of amelogenesis. The efflux of ameloblasts, stratum intermedium and papillary layer cells from the presecretory zone was about equal to the efflux plus expected growth within the secretory zone. However, between the secretory and maturation zones about 50% more ameloblasts entered the maturation zone than were required to account for the egress at the gingival margin and the expected growth. Since there was no similar imbalance between these zones for papillary layer cells, it was concluded that this discrepancy must represent a 50% reduction in the size of the ameloblast population during the maturation stage of amelogenesis. A little over 25% of the loss occurred immediately at the start of maturation within the region of postsecretory transition and the remaining 25% of the loss occurred throughout the subsequent regions of the maturation zone. In addition to the kinetic analysis, graphic reconstructions, or surface maps, of ameloblast nuclei were prepared. These maps illustrated the characteristics of ameloblast nuclear packing within the 3 zones of amelogenesis and they provided quantitative confirmation that as ameloblasts progress through the maturation zone, there is a loss of cells in an amount predicted by the kinetic analysis.