Corticotropin-releasing hormone system in human adipose tissue.
Corticotropin-releasing hormone system in human adipose tissue.
复制标题
人体脂肪组织中的促肾上腺皮质激素释放激素系统。
DOI：
10.1210/jc.2003-031299
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Ehrhart‐Bornstein
中科院分区：
文献类型：
--
作者：
J. Šereš;S. Bornstein;P. Seres;H. Willenberg;K. Schulte;W. Scherbaum;M. Ehrhart‐Bornstein
越来越多的证据表明CRH系统在能量平衡中的作用，包括对人类脂肪细胞的直接影响，脂肪11 β -羟基类固醇脱氢酶1型活性的调节，以及皮质醇的形成。与其他外周组织、肾上腺和心脏相比，我们表征了CRH受体1和2以及CRH样肽应激蛋白和尿皮质素在人脂肪组织中的表达。采用定量TaqMan PCR分析CRH对人离体脂肪细胞中CRH受体及CRH样肽表达的影响。CRH受体在脂肪组织中mRNA和蛋白水平均可检测到。CRH-R2在脂肪中的表达与在心脏中的表达相当，心脏是已知CRH-R2表达最高的器官。CRH-R1:CRH-R2比值随脂肪库类型的不同而不同；CRH-R1在sc脂肪中的表达高于内脏脂肪，而CRH-R2的表达则相反。脂肪组织也表达外周CRH-R2的主要配体——尿皮质素和应激蛋白。CRH下调离体脂肪细胞中CRH- r1和CRH- r2 mRNA的表达。这些数据，加上最近发表的CRH通过下调11 β -羟基类固醇脱氢酶调节脂肪细胞代谢的观察结果，表明CRH系统存在于人类脂肪组织中。该系统可能与能量稳态有关，并在脂肪水平介导CRH的厌食作用。
Mounting evidence exists for a role of the CRH system in energy balance, including a direct influence on human adipocytes, the regulation of adipose 11 beta-hydroxysteroid dehydrogenase type 1 activity, and cortisol formation. We characterized the expression of CRH receptors 1 and 2 and CRH-like peptides stresscopin and urocortin in human adipose tissue in comparison with other peripheral tissues, adrenal, and heart. The effect of CRH on CRH receptor and CRH-like peptide expression was analyzed in isolated human adipocytes using quantitative TaqMan PCR. CRH receptors were detectable in fat tissue at mRNA and protein levels. CRH-R2 expression in fat was comparable with its expression in the heart, the organ with the highest CRH-R2 expression known. CRH-R1:CRH-R2 ratio varied according to fat-depot type; whereas CRH-R1 expression was higher in sc fat than in visceral fat, the opposite was true for CRH-R2. Adipose tissue also expressed urocortin and stresscopin, the predominant ligands of peripheral CRH-R2. CRH down-regulated CRH-R1 and CRH-R2 mRNA expression in isolated adipocytes. These data, together with the recently published observation that CRH regulates adipocyte metabolism by down-regulating 11 beta-hydroxysteroid dehydrogenase, indicate that a CRH system exists within human adipose tissue. This system could be implicated in energy homeostasis and in mediating the anorexic effects of CRH at adipose level.
影响因子：
5.8
作者：
Friedberg, M;Zoumakis, E;Hochberg, Z
通讯作者：
Hochberg, Z
影响因子：
4.7
作者：
Tsigos, C;Chrousos, GP
通讯作者：
Chrousos, GP