Lettuce and rhizosphere microbiome responses to growth promoting Pseudomonas species under field conditions
Lettuce and rhizosphere microbiome responses to growth promoting Pseudomonas species under field conditions
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DOI：
10.1093/femsec/fiw197
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发表时间：
2016-12-01
影响因子：
4.2
通讯作者：
Kuramae, Eiko E.
中科院分区：
文献类型：
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作者：
Cipriano, Matheus A. P.;Lupatini, Manoeli;Kuramae, Eiko E.
促进植物生长的根际细菌已被充分描述并推荐用于世界范围内的多种作物。然而，研究中最常见的问题之一是难以获得可重复的结果。此外，很少有研究评估田间条件下细菌接种对植物生长的促进作用和土壤微生物群落组成。在这里，我们评估了 54 种假单胞菌菌株对生菜 (Lactuca sativa) 生长的影响。对 12 种最有前途的菌株进行了系统发育和生理学特征的植物生长促进性状，包括磷酸盐溶解、激素产生和对病原体化合物的拮抗作用，以及它们在农田条件下对植物生长的影响。此外，还评估了接种植物的有益菌株对根际细菌群落的影响。菌株IAC-RBcr4和IAC-RBru1具有不同的植物促生长性状，可将生菜植物生物量产量提高高达30%。这两种菌株还影响根际细菌群，包括 Isosphaera 和 Pirellula（浮霉菌门）以及 Acidothermus、Pseudolabrys 和 Singusphaera（放线菌门）。这是第一项证明假单胞菌菌株对热带田间条件下生长的生菜幼苗和植物生长促进作用的一致结果的研究。
Plant growth promoting rhizobacteria are well described and recommended for several crops worldwide. However, one of the most common problems in research into them is the difficulty in obtaining reproducible results. Furthermore, few studies have evaluated plant growth promotion and soil microbial community composition resulting from bacterial inoculation under field conditions. Here we evaluated the effect of 54 Pseudomonas strains on lettuce (Lactuca sativa) growth. The 12 most promising strains were phylogenetically and physiologically characterized for plant growth-promoting traits, including phosphate solubilization, hormone production and antagonism to pathogen compounds, and their effect on plant growth under farm field conditions. Additionally, the impact of beneficial strains on the rhizospheric bacterial community was evaluated for inoculated plants. The strains IAC-RBcr4 and IAC-RBru1, with different plant growth promoting traits, improved lettuce plant biomass yields up to 30%. These two strains also impacted rhizosphere bacterial groups including Isosphaera and Pirellula (phylum Planctomycetes) and Acidothermus, Pseudolabrys and Singusphaera (phylum Actinobacteria). This is the first study to demonstrate consistent results for the effects of Pseudomonas strains on lettuce growth promotion for seedlings and plants grown under tropical field conditions.