Identification of a regulatory transposon that controls the Mutator transposable element system in maize.
Identification of a regulatory transposon that controls the Mutator transposable element system in maize.
复制标题
鉴定控制玉米突变转座元件系统的调节转座子。
DOI：
10.1093/genetics/129.1.261
复制
发表时间：
1991
期刊：
影响因子：
3.3
通讯作者：
Freeling,M
中科院分区：
文献类型：
--
作者：
Chomet,P;Lisch,D;Hardeman,KJ;Chandler,VL;Freeling,M
玉米突变体系统由八种以上不同类别的转座元件组成，每一种转座元件都可以在多个副本中找到。所有Mu元素共享大约220个碱基的末端反向重复序列，而每个不同的元素类由其独特的内部序列定义。由于该系统的多基因遗传，其不稳定性的调节一直难以阐明。在这里，我们介绍的遗传学实验证明，存在一个单一的基因座，MuR1，它可以调节MU1元件的转座。我们描述了一类新的Mu元件MUR的成员的克隆，并证明了这一类的成员是突变体活性的调节器MuR1。这一结论基于几个标准：MuR1活性和MuR同源限制性片段共分离；当MuR1经历复制转座时，观察到额外的MUR限制片段，并且MuR1活性和共同分离的MUR片段同时在克隆体细胞扇区丢失。此外，在具有突变子活性的植物中，mur元件与转录本杂交。我们的遗传实验表明，MuR1转座子是我们品系中指定突变体活性所必需的。
The Mutator system of maize consists of more than eight different classes of transposable elements each of which can be found in multiple copies. All Mu elements share the approximately 220-bp terminal inverted repeats, whereas each distinct element class is defined by its unique internal sequences. The regulation of instability of this system has been difficult to elucidate due to its multigenic inheritance. Here we present genetic experiments which demonstrate that there is a single locus, MuR1, which can regulate the transposition of Mu1 elements. We describe the cloning of members of a novel class of Mu elements, MuR, and demonstrate that a member of the class is the regulator of Mutator activity, MuR1. This conclusion is based on several criteria: MuR1 activity and a MuR-homologous restriction fragment cosegregate; when MuR1 undergoes a duplicative transposition, an additional MuR restriction fragment is observed, and MuR1 activity and the cosegregating MuR fragment are simultaneously lost within clonal somatic sectors. In addition, the MuR element hybridizes to transcripts in plants with Mutator activity. Our genetic experiments demonstrate that the MuR1 transposon is necessary to specify Mutator activity in our lines.