Assessment of genetic relationships among cultivated and wild Rubusaccessions using AFLP markers
Assessment of genetic relationships among cultivated and wild Rubusaccessions using AFLP markers
复制标题
使用 AFLP 标记评估栽培和野生悬钩子种质之间的遗传关系
DOI：
10.1016/j.scienta.2015.07.004
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发表时间：
2015
影响因子：
4.3
通讯作者：
Yoichiro Hoshino
中科院分区：
文献类型：
--
作者：
Tomoya Miyashita;Hisato Kunitake;Norishige Yotsukura;Yoichiro Hoshino
悬钩子属包括740多个物种，分为12个亚属，包含乌藨子，黑莓，北极果和开花乌藨子，所有这些都被用于育种计划。本研究的目的是评价主要在日本采集的野生和栽培悬钩子属物种之间的系统发育关系。为评价悬钩子属植物的遗传资源，采用3对AFLP引物对81份悬钩子属植物进行了遗传多样性分析，并采用邻位连接法和算术平均数加权对组法对数据进行了分析。两个生成的系统发育树分组亚属Anoplobatus，Eubatus，Shakespeeobatus，和Malachobatus到不同的集群。标题悬钩子的种质资源。从北海道的4个地区采集到的刺叶松（var. aculeatissimus）形成了反映采样地点的明显聚类。以4个树莓品种（R. idaeusL.）Andr. idaeusL. var.aculeatissimus和一个树莓品种和悬钩子之间的杂种加入明显区别于亲本加入。这些结果表明AFLP标记是评价悬钩子属植物遗传多样性和系统发育关系的可靠技术。从多样性和系统发育研究的数据揭示了宝贵的信息，在不同的加入，这将是有用的，通过传统的育种和标记辅助选择的改进基因型的发展的独特片段的可用性。
The genusRubuscomprises more than 740 species divided into 12 subgenera and contains the raspberries, blackberries, arctic fruits, and flowering raspberries, all of which have been utilized in breeding programs. The objective of this study was to evaluate the phylogenetic relationships among wild and cultivatedRubusspecies mainly collected in Japan. To evaluate genetic resources inRubus, 81 accessions were analyzed with three amplified fragment length polymorphism (AFLP) primer pairs and data were analyzed with the neighbor-joining and unweighted pair group methods with arithmetic mean. Two of the generated phylogenetic trees grouped subgeneraAnoplobatus,Eubatus,Idaeobatus, andMalachobatusinto different clusters. Accessions ofRubus idaeusL. var.aculeatissimuscollected from four regions in Hokkaido formed distinct clusters reflecting sampling sites. Four hybrid accessions between raspberry cultivars (R. idaeusL.) andR. idaeusL. var.aculeatissimus, and one hybrid accession between a raspberry cultivar andRubus spectabilisPursh were clearly distinguished from parental accessions. These results indicated that AFLP markers are a reliable technique for assessing genetic diversity and studying phylogenetic relationships inRubus. Data from diversity and phylogenetic studies revealed valuable information on the availability of unique fragments in different accessions that would be useful for the development of improved genotypes through conventional breeding and marker-assisted selection.