Streptococcus canis multilocus sequence typing in a case series of dogs with ulcerative keratitis
Streptococcus canis multilocus sequence typing in a case series of dogs with ulcerative keratitis
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DOI：
10.1111/vop.12714
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发表时间：
2019-09-30
影响因子：
1.6
通讯作者：
Waller, Andrew S.
中科院分区：
文献类型：
--
作者：
Enache, Andra E.;Mitchell, Catriona;Waller, Andrew S.
目的：确定从动物健康信托基金 (AHT) 诊断为溃疡性角膜炎的狗身上回收的四种犬链球菌 (Scanis) 分离株是否与在线数据库中注册的其他眼部分离株有遗传相关性。研究动物：四种 Scanis 角膜分离株。程序：检索 2016 年至 2017 年间的临床和实验室记录，寻找患有溃疡性角膜炎的狗，其微生物学分析与犬链球菌的生长情况一致。斯卡尼斯.提取基因组 DNA 进行测序 (Illumina MiSeq)，并使用 MLST 1.8 相对于 Scanis 的 44 种可用序列类型确定多位点序列类型 (ST)。 MEGA v4.0 中构建了邻居连接树。使用双边费舍尔精确检验来确定分离的菌株与狗的眼部感染之间的任何关联。结果：从患有溃疡性角膜炎的哈巴狗（病例1-4）中分离出四种菌株。基因组测序鉴定出 ST-27（病例 1）、ST-9（病例 3）和 ST-13（病例 2 和 4）。 ST 13 和 27 是克隆复合体 (CC)-13 的成员。对多位点序列分型数据库的分析显示，从狗的眼部分泌物中回收的 12 种分离株中，CC-13 菌株占 6 种 (P=.0078)。 结论：早期证据表明 Scanis 的 CC-13 组与狗的眼部感染有关。我们提供基因组序列草案，以供未来鉴定与狗链球菌性角膜炎相关的毒力机制。
Objective: To determine whether four isolates of Streptococcus canis (Scanis) recovered from dogs diagnosed with ulcerative keratitis at the Animal Health Trust (AHT) were genetically related to other ocular isolates that are registered in the online database.Animal studied: Four Scanis corneal isolates.Procedures: Clinical and laboratory records between 2016 and 2017 were searched for dogs with ulcerative keratitis for which microbiology analysis was consistent with the growth of Scanis. Genomic DNA was extracted for sequencing (Illumina MiSeq), and multilocus sequence types (STs) were determined using MLST 1.8 relative to the 44 sequence types of Scanis available. A neighbor-joining tree was constructed in MEGA v4.0. A two-sided Fisher's exact test was used to determine any associations between the isolated strains and ocular infections of dogs.Results: Four strains were isolated from pugs (cases 1-4) with ulcerative keratitis. Genome sequencing identified ST-27 (case 1), ST-9 (case 3), and ST-13 (cases 2 and 4). STs 13 and 27 are members of Clonal Complex (CC)-13. Analysis of the multilocus sequence typing database revealed that CC-13 strains accounted for six of the twelve isolates recovered from the eye exudates of dogs (P=.0078).Conclusions: There is early evidence that the CC-13 group of Scanis is associated with ocular infections in dogs. We provide draft genome sequences toward the future identification of virulence mechanisms associated with streptococcal keratitis in dogs.