C-type natriuretic peptides are potent dilators of shark vascular smooth muscle.
C-type natriuretic peptides are potent dilators of shark vascular smooth muscle.
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C型利钠肽是鲨鱼血管平滑肌的有效扩张剂。
DOI：
10.1002/jez.1402650112
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
ForrestJr,JN
中科院分区：
文献类型：
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作者：
Evans,DH;Toop,T;Donald,J;ForrestJr,JN
与其他利钠肽相比，先前在完整哺乳动物中对C型利钠肽（CNP）作用的研究显示了有限的利尿反应。我们之前的研究，从各种鱼类分离的血管平滑肌（VSM），利用哺乳动物或非同源的鱼心钠素（ANP），已经证明了血管舒张具有相对较高的灵敏度（EC50 ≤ 5 nM）。最近对角鲨（Squalus acanthias）心脏中的C型利钠肽进行了测序，使我们能够将该肽对该物种主动脉VSM的疗效与其他两种CNP（来自鳉鱼和猪）以及大鼠ANP进行比较。对sCNP、kCNP和pCNP的扩张EC 50为0.5 nM，比对rANP的反应EC 50低15倍以上。这些数据表明，CNP从角鲨心脏释放，是一种具有强效血管舒张作用的循环激素，与CNP在哺乳动物中主要作为脑神经肽的明显作用形成鲜明对比。© 1993 Wiley利斯公司
Previous studies of the effects of C‐type natriuretic peptides (CNP) in intact mammals have demonstrated limited hypotensive responses, in contrast to other natriuretic peptides. Our previous studies, on isolated vascular smooth muscle (VSM) from various fish species, utilizing either mammalian or non‐homologous fish atrial natriuretic peptides (ANP), have demonstrated vasodilation with a relatively high sensitivity (EC50∼ 5 nM). The recent sequencing of a C‐type natriuretic peptide from the heart of the dogfish shark,Squalus acanthias, has enabled us to compare the efficacy of this peptide on aortic VSM from that species with two other CNPs (from killifish and pig), as well as rat ANP. The EC50of dilation for sCNP, as well as kCNP and pCNP, was 0.5 nM, over 15 times lower than the EC50of the response to rANP. These data suggest that CNP is released from the dogfish shark heart and is a circulating hormone with potent vasodilatory effects, in sharp contrast to the apparent role of CNP predominantly as a brain neuropeptide in mammals. © 1993 Wiley‐Liss, Inc.