Heat Shock Procedure Affects Cell Division‑Associated Genes in Gynogenetic Manipulation
Heat Shock Procedure Affects Cell Division‑Associated Genes in Gynogenetic Manipulation
复制标题
热激程序影响细胞分裂——雌核遗传操作中的相关基因
DOI：
10.1007/s10126-022-10112-4
复制
发表时间：
2022
影响因子：
3
通讯作者：
Fan Yu
中科院分区：
文献类型：
--
作者：
Fan Yu
热休克过程是雌核发育操作的关键，导致母本基因组二倍体化，但其分子机制，特别是转录组学的变化，在这一过程中仍然没有得到揭示。本研究利用稀有鲫（Gobiocypris rarus）的灭活精子进行斑马鱼（Danio rerio）的人工雌核发育。我们发现，人工雌核发育操作，包括假受精和热休克，孵化率下降，而单独热休克处理有中等孵化率。第一次切割改变了与RNA转录和蛋白质合成相关的基因的表达。由中枢基因GIT 1、Sepsecs和FLNB调控的共表达网络与热休克过程显著相关。细胞周期蛋白家族和细胞周期蛋白依赖性激酶相关基因在雌核发育斑马鱼胚胎中低表达，而控制细胞周期和基因组稳定性的基因被雌核发育处理显著改变。我们的研究结果显示了人工雌核发育对胚胎的影响，并描述了基因表达的变化，表明热休克过程中细胞分裂发生了剧烈的变化。这些发现有助于进一步了解植物种质改良的分子基础，包括雌核发育的净化作用和异育雌核发育的生物学效应。
Heat shock procedure is crucial for gynogenetic manipulation leading to diploidization of the maternal genomes; however, the underlying molecular mechanism especially the transcriptomic changes during this procedure has still not been unveiled yet. Here, the artificial gynogenesis of zebrafish (Danio rerio) using inactivated sperm from rare minnow (Gobiocypris rarus) was conducted. We found that artificial gynogenetic manipulation, including pseudo-fertilization and heat shock, decreased hatching rates, whereas heat shock treatment alone had medium hatching rates. The first cleavage changed the expression of genes associated with RNA transcription and protein synthesis. A co-expression network regulated by hub genesGIT1,Sepsecs, andFLNBwas significantly correlated with heat shock procedure. The cyclin family and cyclin-dependent kinase-related genes were lowly expressed in embryos from gynogenetic zebrafish, and genes involved in controlling the cell cycle and genomic stability were significantly altered by the gynogenetic treatment. Our results show the effects of artificial gynogenesis on embryos and describe changes in gene expression that suggest drastic changes take place in cell division by heat shock procedure. These findings will contribute to an understanding of the molecular basis for germplasm improving, including the purifying effect and allogynogenetic biological effect by gynogenesis.