Proinflammatory responses to lipo-oligosaccharide of Neisseria meningitidis immunotype strains in relation to virulence and disease
Proinflammatory responses to lipo-oligosaccharide of Neisseria meningitidis immunotype strains in relation to virulence and disease
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DOI：
10.1086/340501
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发表时间：
2002-05-15
影响因子：
6.4
通讯作者：
Beuth, J
中科院分区：
文献类型：
--
作者：
Braun, JM;Blackwell, CC;Beuth, J
对脂寡糖（LOS）的炎症反应有助于脑膜炎球菌病的严重性。表达L（3，7，9）LOS免疫型的菌株分离自大多数患者，但其他免疫型主要分离自携带者。在用来自标准免疫型菌株的纯化LOS刺激后，比较了用维生素D3（VD 3）预处理的人单核细胞系（THP-1）引起的炎症反应。比较了正常人血清和B：2a：P1.5，2：L3株免疫小鼠血清的中和效果。与其他免疫型相比，L3、L7、L 8和L9免疫型的LOS诱导了显著更高水平的肿瘤坏死因子-α和白细胞介素-6。正常人血清中和了对所有测试的免疫型的LOS的促炎反应。免疫小鼠血清中和针对来自具有与L（3，7，9）部分交叉反应的表位的免疫型的LOS的炎症应答。在正常人血清和免疫小鼠血清中发现的脑膜炎球菌内毒素寡糖、核心和脂质A部分的抗体有助于中和活性。
Inflammatory responses to lipo-oligosaccharide (LOS) contribute to the severity of meningococcal disease. Strains that express the L(3,7,9) LOS immunotypes are isolated from the majority of patients, but other immunotypes are isolated predominantly from carriers. Inflammatory responses elicited from a human monocytic cell line (THP-1) that had been pretreated with vitamin D3 (VD3) were compared after stimulation with purified LOSs from standard immunotype strains. The neutralizing effects of normal human serum and serum from mice immunized with strain B:2a:P1.5,2:L3 were compared. LOSs of immunotypes L3, L7, L8, and L9 induced significantly higher levels of tumor necrosis factor-alpha and interleukin-6, compared with other immunotypes. Normal human serum neutralized the proinflammatory responses to LOSs of all immunotypes tested. Immune mouse serum neutralized inflammatory responses against LOSs from immunotypes with epitopes cross-reactive with L(3,7,9) moieties. Antibodies found in normal human serum and immune mouse serum to the oligosaccharide, core, and lipid A moieties of meningococcal endotoxin contribute to neutralizing activity.