WEAK HLA AND BETA-2-MICROGLOBULIN EXPRESSION OF NEURONAL CELL-LINES CAN BE MODULATED BY INTERFERON
WEAK HLA AND BETA-2-MICROGLOBULIN EXPRESSION OF NEURONAL CELL-LINES CAN BE MODULATED BY INTERFERON
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DOI：
10.1073/pnas.81.20.6476
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
FISHER, CA
中科院分区：
文献类型：
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作者：
LAMPSON, LA;FISHER, CA
先前的研究表明，4种人类神经细胞系中每一种都表达。ltoreq。0.5%的HLA-A，B，C和β。2-微球蛋白见于胶质细胞、淋巴细胞等细胞类型，在成神经细胞瘤肿瘤和成人脑中有相应的弱表达。探讨了这种弱表达的遗传基础。3种神经母细胞瘤细胞系中，HLA-A，B，C和。β。干扰素可以诱导2-微球蛋白，并且这种诱导发生在群体的每个细胞中。II （Ia）类分子未被检测到。完整细胞的显微分析和放射免疫分析表明，诱导抗原出现在细胞表面和每个细胞内。免疫印迹分析证实，诱导蛋白具有I类分子结构。因此，正常的弱HLA和。这些细胞系的2-微球蛋白表达似乎反映了一种调节控制，而不是原发性遗传病变。根据目前的理论，缺乏HLA-A，B，C应该保护转化，感染或损伤的神经元，以及神经移植中的神经元免受T细胞介导的免疫监视。在这种情况下，神经元HLA-A，B，C表达可能受到调节控制的可能性是重要的。
Previous work showed that each of 4 human neuronal cell lines expresses .ltoreq. 0.5% of the HLA-A,B,C and .beta.2-microglobulin seen in glial, lymphoid and other cell types, and there is a corresponding weak expression in neuroblastoma tumor and adult brain. The genetic basis of this weak expression was probed. For each of 3 neuroblastoma cell lines, it was shown that HLA-A,B,C and .beta.2-microglobulin can be induced by interferon and that the induction occurs within every cell of the population. Class II (Ia) molecules are not detected. Microscopic assay and radioimmunoassay of intact cells suggest that the induced antigen appears to the cell surface as well as within each cell. Immunoblot analysis confirms that the induced proteins have the structure of class I molecules. Thus, the normal weak HLA and .beta.2-microglobulin expression of these cell lines appears to reflect a regulatory control rather than a primary genetic lesion. According to current theory, lack of HLA-A,B,C should protect transformed, infected or damage neurons, but also neurons in neural transplants, from T cell-mediated immunosurveillance. The possibility that neuronal HLA-A,B,C expression may be under regulatory control is of importance in this context.